Wipf Juliette R K, Schwendener Sybille, Nielsen Jesper Boye, Westh Henrik, Perreten Vincent
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Antimicrob Agents Chemother. 2015;59(6):3578-81. doi: 10.1128/AAC.00369-15. Epub 2015 Mar 16.
Genome alignment of a macrolide, lincosamide, and streptogramin B (MLSB)-resistant Staphylococcus fleurettii strain with an MLSB-susceptible S. fleurettii strain revealed a novel 11,513-bp genomic island carrying the new erythromycin resistance methylase gene erm(45). This gene was shown to confer inducible MLSB resistance when cloned into Staphylococcus aureus. The erm(45)-containing island was integrated into the housekeeping gene guaA in S. fleurettii and was able to form a circular intermediate but was not transmissible to S. aureus.
对一株对大环内酯类、林可酰胺类和链阳菌素B（MLSB）耐药的弗勒里葡萄球菌菌株与一株对MLSB敏感的弗勒里葡萄球菌菌株进行基因组比对，发现了一个新的11513碱基对的基因组岛，该岛携带新的红霉素抗性甲基化酶基因erm(45)。当该基因克隆到金黄色葡萄球菌中时，显示出可诱导的MLSB抗性。含有erm(45)的岛整合到弗勒里葡萄球菌的管家基因guaA中，能够形成环状中间体，但不能传递给金黄色葡萄球菌。
