Quirke P, Dyson J E, Dixon M F, Bird C C, Joslin C A
Br J Cancer. 1985 Jan;51(1):99-106. doi: 10.1038/bjc.1985.14.
Flow cytometry and histopathology were utilised in evaluating 50 primary and 16 metastatic colorectal carcinomas to determine the influence of heterogeneity and proportion of dying cells on pathological assessments. A new procedure was developed for staining unfixed whole cells with acridine orange and ethidium bromide to quantify DNA and RNA content and number of dead and dying cells. Attempts were made to reduce interobserver variation in histological assessment and to determine whether flow cytometry could refine current grading and staging procedures. Interobserver variation in grading was not improved by estimating proportions of differing grades in multiple samples from individual tumours. Considerable heterogeneity was observed within tumours although this was less apparent when defining ploidy status than histological grade. No consistent differences were observed between superficial and deep parts of tumours or between primary and secondary tumours by either method of analysis. The proportion of dead and dying cells varied widely between tumours but there was no correlation with tumour grade or stage. Non-diploid tumours were not of more advanced stage or poorer histological grade than diploid tumours. Since ploidy status may be an important prognostic factor, analysis of colorectal carcinomas by flow cytometry could be of greater value than conventional grading and staging procedures.
采用流式细胞术和组织病理学方法对50例原发性和16例转移性结直肠癌进行评估，以确定异质性和死亡细胞比例对病理评估的影响。开发了一种用吖啶橙和溴化乙锭对未固定的全细胞进行染色的新方法，以量化DNA和RNA含量以及死亡和濒死细胞的数量。试图减少组织学评估中的观察者间差异，并确定流式细胞术是否能改进当前的分级和分期程序。通过估计单个肿瘤多个样本中不同分级的比例，观察者间在分级上的差异并未得到改善。肿瘤内观察到相当大的异质性，尽管在定义倍体状态时不如组织学分级明显。通过两种分析方法，在肿瘤的浅表和深部之间或原发性和继发性肿瘤之间均未观察到一致的差异。肿瘤之间死亡和濒死细胞的比例差异很大，但与肿瘤分级或分期无关。非二倍体肿瘤并不比二倍体肿瘤处于更晚期或组织学分级更差。由于倍体状态可能是一个重要的预后因素，通过流式细胞术分析结直肠癌可能比传统的分级和分期程序更有价值。
