多灶同步性肝细胞癌中DNA含量的异质性
Heterogeneity of DNA content in multiple synchronous hepatocellular carcinomas.
作者信息
Hui A M, Kawasaki S, Imamura H, Miyagawa S, Ishii K, Katsuyama T, Makuuchi M
机构信息
First Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
出版信息
Br J Cancer. 1997;76(3):335-9. doi: 10.1038/bjc.1997.387.
Heterogeneity of DNA content in multiple hepatocellular carcinomas (HCCs) was investigated by flow cytometry in 62 tumours from 26 patients who had undergone surgical treatment for multiple synchronous HCCs. Heterogeneity of DNA content was defined (a) when tumours had a different DNA ploidy pattern or (b) when the difference in the DNA index of the aneuploid clone was more than 0.1. A tumour with DNA aneuploidy was observed in 17 (66%) of the 26 patients. Heterogeneity of the DNA content was demonstrated in 12 (46%) out of 26 patients: in ten cases by definition (a) and in two cases by definition (b). Histological examination revealed that, of the 12 patients with a heterogeneous tumour DNA content, seven (58%) had a heterogeneous and the remaining five (42%) had a homogeneous type and grade of differentiation among the tumours, showing the absence of a relationship between histological heterogeneity and DNA content. The present results suggest the clinical relevance of DNA content analysis for identifying the clonal origin of multiple HCCs.
通过流式细胞术对26例接受多灶性同步肝细胞癌（HCC）手术治疗患者的62个肿瘤进行研究，以探讨多灶性HCC中DNA含量的异质性。DNA含量异质性定义为：（a）肿瘤具有不同的DNA倍体模式，或（b）非整倍体克隆的DNA指数差异大于0.1。26例患者中有17例（66%）观察到DNA非整倍体肿瘤。26例患者中有12例（46%）表现出DNA含量异质性：10例符合定义（a），2例符合定义（b）。组织学检查显示，在12例肿瘤DNA含量异质性患者中，7例（58%）肿瘤间分化类型和分级存在异质性，其余5例（42%）为同质性，表明组织学异质性与DNA含量之间不存在相关性。目前的结果提示DNA含量分析对于确定多灶性HCC的克隆起源具有临床意义。
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