Gershon R K, Kondo K
Immunology. 1970 May;18(5):723-37.
Thymectomized, lethally irradiated, bone marrow reconstituted mice were treated with a large dose of sheep red blood cells (SRBC) over the course of 30 days. They were unable to respond to further antigenic challenge for one month. Fifteen million thymocytes given 4 days after the termination of treatment restored their ability to respond. The same antigenic treatment given to similar chimeras, which differed only in having had 15 × 10 thymus cells added to the bone marrow inoculum, also abolished the response to further antigenic challenge. In contrast to chimeras without thymus cells present during the course of treatment, the later addition of thymocytes to these animals did not restore their response. It did, however, restore the response to a second challenge of antigen given 17 days after the addition of thymocytes. This response was the same as non-treated animals given only one injection of thymocytes and significantly less than non-treated animals given thymocytes twice. The following explanation of these results is offered. Bone marrow derived (BMD) lymphocytes that can make antibody without assistance of thymus derived (TD) lymphocytes were made tolerant in the absence of TD cells. Thymus dependent BMD cells were not. New cells, coming from the bone marrow, broke the tolerant state within a month. When TD cells were present both populations of BMD cells, as well as the TD cells, were made tolerant. New BMD cells regenerating from the bone marrow abrogated the tolerant state of the BMD population. This breaking of tolerance could only be seen in mice given additional thymocytes as the tolerance of the TD cells was not broken in the absence of a thymus. Thus, the induction of tolerance as well as the induction of immunity in thymus dependent BMD cell populations, seems to require the co-operation of TD cells.
对经过胸腺切除、致死剂量照射并进行骨髓重建的小鼠，在30天内给予大剂量绵羊红细胞（SRBC）。它们在一个月内无法对进一步的抗原刺激作出反应。治疗结束4天后给予1500万个胸腺细胞可恢复其反应能力。对类似的嵌合体进行相同的抗原处理，这些嵌合体的唯一区别在于在骨髓接种物中添加了15×10个胸腺细胞，这也消除了对进一步抗原刺激的反应。与在治疗过程中没有胸腺细胞的嵌合体相比，后来给这些动物添加胸腺细胞并没有恢复它们的反应。然而，在添加胸腺细胞17天后给予第二次抗原刺激时，确实恢复了反应。这种反应与只注射一次胸腺细胞的未处理动物相同，且明显低于注射两次胸腺细胞的未处理动物。对这些结果给出了以下解释。在没有胸腺来源（TD）淋巴细胞协助的情况下能够产生抗体的骨髓来源（BMD）淋巴细胞在没有TD细胞时产生了耐受性。胸腺依赖性BMD细胞则没有。来自骨髓的新细胞在一个月内打破了耐受状态。当TD细胞存在时，BMD细胞群体以及TD细胞都产生了耐受性。从骨髓再生的新BMD细胞消除了BMD群体的耐受状态。只有在给予额外胸腺细胞的小鼠中才能看到这种耐受性的打破，因为在没有胸腺的情况下TD细胞的耐受性没有被打破。因此，胸腺依赖性BMD细胞群体中耐受性的诱导以及免疫的诱导似乎都需要TD细胞的合作。
