Kimber I, Shepherd C J, Mitchell J A, Turk J L, Baker D
Immunology Group, Central Toxicology Laboratory ICI plc, Macclesfield, Cheshire, U.K.
Immunology. 1989 Apr;66(4):577-82.
Epicutaneous exposure of mice to the contact sensitizing chemicals 4-ethoxymethylene-2-phenyl-oxazol-5-one (oxazolone) and 2,4,6-trinitrochlorobenzene (picryl chloride) causes an inhibition of proliferative responses induced following subsequent topical challenge. The effects on lymphocyte proliferation comprise both transient antigen non-specific and more persistent hapten-specific mechanisms. Pretreatment of mice with one chemical 5 days prior to sensitization with a second, at which time antigen non-specific influences on proliferative responses are manifest, results in depression of contact sensitization as measured by changes in ear thickness following challenge. If, however, the period between pretreatment and sensitization is extended the inhibition of contact sensitization disappears in parallel with a decline in the antigen non-specific depression of lymph node cell proliferation. These data reveal that there exist two homeostatic mechanisms which control proliferation in response to challenge with at least some antigens, and that the extent of lymphocyte proliferation directly influences the degree of contact sensitization achieved. Moreover these results demonstrate that, in some instances at least, competition between antigens may be a function of immunoregulatory influences on lymphocyte proliferation.
将小鼠经皮暴露于接触致敏化学物质4-乙氧基亚甲基-2-苯基恶唑-5-酮（恶唑酮）和2,4,6-三硝基氯苯（苦味酰氯），会抑制随后局部激发后诱导的增殖反应。对淋巴细胞增殖的影响包括短暂的抗原非特异性机制和更持久的半抗原特异性机制。在用第二种化学物质致敏前5天用一种化学物质预处理小鼠，此时抗原对增殖反应的非特异性影响明显，结果显示，通过激发后耳部厚度的变化测量，接触致敏受到抑制。然而，如果预处理和致敏之间的时间间隔延长，接触致敏的抑制作用会消失，同时淋巴结细胞增殖的抗原非特异性抑制作用也会下降。这些数据表明，存在两种稳态机制来控制对至少某些抗原激发的增殖反应，并且淋巴细胞增殖的程度直接影响所实现的接触致敏程度。此外，这些结果表明，至少在某些情况下，抗原之间的竞争可能是免疫调节对淋巴细胞增殖影响的一种功能。
