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Si-CSP9调节入侵红火蚁（Solenopsis invicta）幼虫的体壁和蜕皮过程。

Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta.

作者信息

Cheng Daifeng, Lu Yongyue, Zeng Ling, Liang Guangwen, He Xiaofang

机构信息

Department of Entomology, South China Agricultural University, Guangzhou, Guangdong, People's Republic China.

出版信息

Sci Rep. 2015 Mar 18;5:9245. doi: 10.1038/srep09245.

DOI:10.1038/srep09245
PMID:25784646
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4363891/
Abstract

Chemosensory proteins (CSPs) have been predicted to be involved in development; however, direct evidence for their involvement is lacking, and genetic basis is largely unknown. To determine the function of the chemosensory protein 9 (Si-CSP9) gene in Solenopsis invicta, we used RNA interference to silence Si-CSP9 in 3rd-instar larvae. The 3rd-instar larvae failed to shed their cuticle after being fed Si-CSP9-directed siRNA, and expression profiling of RNAi-treated and untreated control larvae showed that 375 genes were differentially expressed. Pathway enrichment analysis revealed that 4 pathways associated with larval development were significantly enriched. Blast analysis revealed that one fatty acid amide hydrolase (FAAH) gene was up-regulated and 4 fatty acid synthase (FAT) genes and one protein kinase DC2 gene (PKA) were down-regulated in the enriched pathways. Significantly higher expression of these genes was found in 4th-instar larvae, and Pearson correlation analysis of the expression patterns revealed significant relationships among Si-CSP9, PKA, FAAH, and FAT1-4. Moreover, we confirmed that expression levels of Si-CSP9, FAAH, and FAT1-4 were significantly reduced and that the development of 3rd-instar larvae was halted with PKA silencing. These results suggest that Si-CSP9 and PKA may be involved in the network that contributes to development of 3rd-instar larvae.

摘要

化学感受蛋白（CSPs）被预测参与发育过程；然而，缺乏其参与的直接证据，且遗传基础很大程度上未知。为了确定红火蚁中化学感受蛋白9（Si-CSP9）基因的功能，我们使用RNA干扰技术在三龄幼虫中沉默Si-CSP9。喂食Si-CSP9靶向siRNA后，三龄幼虫无法蜕去表皮，对RNAi处理和未处理的对照幼虫进行表达谱分析表明，有375个基因表达存在差异。通路富集分析显示，4条与幼虫发育相关的通路显著富集。Blast分析显示，在富集通路中，一个脂肪酸酰胺水解酶（FAAH）基因上调，4个脂肪酸合酶（FAT）基因和一个蛋白激酶DC2基因（PKA）下调。在四龄幼虫中发现这些基因的表达显著更高，对表达模式的Pearson相关性分析揭示了Si-CSP9、PKA、FAAH和FAT1-4之间存在显著关系。此外，我们证实，沉默PKA后，Si-CSP9、FAAH和FAT1-4的表达水平显著降低，三龄幼虫的发育停止。这些结果表明，Si-CSP9和PKA可能参与了促进三龄幼虫发育的网络。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/f743071b8a58/srep09245-f13.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/48062b448054/srep09245-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/9f66715f2ace/srep09245-f9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/8228b9bce4e0/srep09245-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/ef843f7e3886/srep09245-f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/f743071b8a58/srep09245-f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/dc5f56a29381/srep09245-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/e19e272d800a/srep09245-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/2ba5039e3389/srep09245-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/818d424d2d71/srep09245-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/7701f7b410be/srep09245-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/66d8490f01cf/srep09245-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/6ae1b3328057/srep09245-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/48062b448054/srep09245-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/9f66715f2ace/srep09245-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/25555fd8c0b1/srep09245-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/8228b9bce4e0/srep09245-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/ef843f7e3886/srep09245-f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/327a/4363891/f743071b8a58/srep09245-f13.jpg

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