Grenho L, Barros J, Ferreira C, Santos V R, Monteiro F J, Ferraz M P, Cortes M E
INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal. I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal. Faculdade de Engenharia, DEMM, Universidade do Porto, Porto, Portugal.
Biomed Mater. 2015 Mar 18;10(2):025004. doi: 10.1088/1748-6041/10/2/025004.
The high number of biomaterial associated infections demands new strategies to prevent this problem. In this study the suitability of nanohydroxyapatite (nanoHA)-based surfaces containing two Brazilian extracts of propolis (green and red ones) to prevent bacterial growth and biofilm formation, as well as its non-cytotoxic nature, was investigated. Optical density, colony forming units and MTT reduction assay were used to assess the materials' antibacterial activity against planktonic and sessile growth of Staphylococcus aureus. NanoHA matrix was able to absorb both types of propolis and the obtained results revealed the antibacterial effectiveness of the novel materials expressed as the reduction of bacterial growth and biofilm formation ability. Additionally, cell culture tests showed the growth of fibroblasts with high metabolic activity and without membrane damage. Therefore, these nanoHA-based surfaces containing natural products deriving from bees may be a promising bioactive biomaterial to be further studied with the aim of application to orthopaedic or dental devices.
大量与生物材料相关的感染需要新的策略来预防这一问题。在本研究中，研究了含有两种巴西蜂胶提取物（绿色和红色）的纳米羟基磷灰石（nanoHA）基表面预防细菌生长和生物膜形成的适用性及其无细胞毒性的特性。使用光密度、菌落形成单位和MTT还原试验来评估材料对金黄色葡萄球菌浮游生长和固着生长的抗菌活性。纳米羟基磷灰石基质能够吸收两种类型的蜂胶，所得结果表明新型材料具有抗菌效果，表现为细菌生长减少和生物膜形成能力降低。此外，细胞培养试验显示成纤维细胞生长且代谢活性高，细胞膜无损伤。因此，这些含有源自蜜蜂的天然产物的纳米羟基磷灰石基表面可能是一种有前景的生物活性生物材料，有待进一步研究以应用于骨科或牙科器械。
