长五聚体蛋白PTX3对脑膜炎奈瑟菌的识别及其作为内源性佐剂的作用。
Recognition of Neisseria meningitidis by the long pentraxin PTX3 and its role as an endogenous adjuvant.
作者信息
Bottazzi Barbara, Santini Laura, Savino Silvana, Giuliani Marzia M, Dueñas Díez Ana I, Mancuso Giuseppe, Beninati Concetta, Sironi Marina, Valentino Sonia, Deban Livija, Garlanda Cecilia, Teti Giuseppe, Pizza Mariagrazia, Rappuoli Rino, Mantovani Alberto
机构信息
Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano (Milan), Italy.
Novartis Vaccines and Diagnostics Research Center, Siena, Italy.
出版信息
PLoS One. 2015 Mar 18;10(3):e0120807. doi: 10.1371/journal.pone.0120807. eCollection 2015.
Long pentraxin 3 (PTX3) is a non-redundant component of the humoral arm of innate immunity. The present study was designed to investigate the interaction of PTX3 with Neisseria meningitidis. PTX3 bound acapsular meningococcus, Neisseria-derived outer membrane vesicles (OMV) and 3 selected meningococcal antigens (GNA0667, GNA1030 and GNA2091). PTX3-recognized microbial moieties are conserved structures which fulfil essential microbial functions. Ptx3-deficient mice had a lower antibody response in vaccination protocols with OMV and co-administration of PTX3 increased the antibody response, particularly in Ptx3-deficient mice. Administration of PTX3 reduced the bacterial load in infant rats challenged with Neisseria meningitidis. These results suggest that PTX3 recognizes a set of conserved structures from Neisseria meningitidis and acts as an amplifier/endogenous adjuvant of responses to this bacterium.
长五聚体蛋白3（PTX3）是天然免疫体液免疫分支中的一个不可或缺的成分。本研究旨在探究PTX3与脑膜炎奈瑟菌之间的相互作用。PTX3可结合无荚膜脑膜炎球菌、源自奈瑟菌的外膜囊泡（OMV）以及3种选定的脑膜炎球菌抗原（GNA0667、GNA1030和GNA2091）。PTX3识别的微生物部分是保守结构，这些结构履行着重要的微生物功能。在使用OMV的疫苗接种方案中，Ptx3基因缺陷型小鼠的抗体反应较低，而同时给予PTX3可增强抗体反应，在Ptx3基因缺陷型小鼠中尤为明显。给予PTX3可降低受到脑膜炎奈瑟菌攻击的幼鼠体内的细菌载量。这些结果表明，PTX3识别脑膜炎奈瑟菌的一组保守结构，并作为针对该细菌反应的增强剂/内源性佐剂发挥作用。
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