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利用针对分离出的血凝素神经氨酸酶（HN）亚基的特异性抗血清对禽副粘病毒的血凝素神经氨酸酶（HN）蛋白进行抗原特性分析。

Antigenic characterization of hemagglutinin-neuraminidase (HN) protein of avian paramyxoviruses by specific antisera to isolated HN subunits.

作者信息

Ishida M, Nerome K, Oya A

出版信息

Arch Virol. 1985;83(3-4):229-39. doi: 10.1007/BF01309919.

DOI:10.1007/BF01309919
PMID:2578784
Abstract

Specific antisera for the isolated HN proteins of eight reference strains of avian paramyxoviruses could be prepared in guinea pigs by intraperitoneal injection of guinea pig red blood cells (GRBC) coated with purified HN proteins. In the hemagglutination inhibition (HI) tests, all reference strains reacted strongly with each homologous antiserum to the isolated HN showing that a low level of cross-reactivity among the reference strains was greatly diminished by using specific antisera. Immuno-double-diffusion (IDD) tests showed that all antisera except those to turkey/Wisconsin/68 and duck/Hong Kong/D3/75 gave single well-defined lines only with the homologous viruses. The remaining two antisera developed a single definite precipitin line together with weak lines with homologous virus. Two isolates in Japan were clearly identified in HI and IDD tests with specific antisera to the HN subunits of the reference strains suggesting that the antisera were useful for identification of avian paramyxovirus isolates. Two isolates in Japan, H-70 from a munia-bird and Y-7 from a duck were found to have HN proteins related closely to those of finch/N. Ireland/Bangor/73 and duck/Hong Kong/199/77, respectively.

摘要

通过腹腔注射包被有纯化HN蛋白的豚鼠红细胞（GRBC），可在豚鼠体内制备针对八种禽副粘病毒参考毒株分离出的HN蛋白的特异性抗血清。在血凝抑制（HI）试验中，所有参考毒株均与针对分离出的HN的各同源抗血清强烈反应，这表明通过使用特异性抗血清，参考毒株之间的低水平交叉反应性大大降低。免疫双扩散（IDD）试验表明，除针对火鸡/威斯康星/68和鸭/香港/D3/75的抗血清外，所有抗血清仅与同源病毒产生单一清晰的沉淀线。其余两种抗血清与同源病毒一起产生一条单一明确的沉淀线以及微弱的沉淀线。在HI和IDD试验中，用针对参考毒株HN亚基的特异性抗血清对日本的两株分离株进行了明确鉴定，这表明这些抗血清可用于鉴定禽副粘病毒分离株。发现日本的两株分离株，即来自梅花雀的H - 70和来自鸭的Y - 7，其HN蛋白分别与雀科鸣禽/北爱尔兰/班戈/73和鸭/香港/199/77的HN蛋白密切相关。

相似文献

1
Antigenic characterization of hemagglutinin-neuraminidase (HN) protein of avian paramyxoviruses by specific antisera to isolated HN subunits.利用针对分离出的血凝素神经氨酸酶（HN）亚基的特异性抗血清对禽副粘病毒的血凝素神经氨酸酶（HN）蛋白进行抗原特性分析。
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引用本文的文献

1
Characterization of reference strains of Newcastle disease virus (NDV) and NDV-like isolates by monoclonal antibodies to HN subunits.
Arch Virol. 1985;85(1-2):109-21. doi: 10.1007/BF01317010.

本文引用的文献

1
Isolation and properties of viruses from poultry in Hong Kong which represent a new (sixth) distinct group of avian paramyxoviruses.从香港家禽中分离出的病毒及其特性，这些病毒代表了禽副粘病毒的一个新的（第六个）独特组别。
J Gen Virol. 1980 Aug;49(2):255-62. doi: 10.1099/0022-1317-49-2-255.
2
Isolation of ortho- and paramyxoviruses from migrating feral ducks in Hokkaido. Brief Report.从北海道迁徙的野鸭中分离出正黏病毒和副黏病毒。简报。
Arch Virol. 1982;74(2-3):211-7. doi: 10.1007/BF01314714.
3
Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies.
用单克隆抗体对N2流感病毒神经氨酸酶的抗原变异体进行筛选和分析。
Virology. 1982 Feb;117(1):93-104. doi: 10.1016/0042-6822(82)90510-4.
4
Mapping of antigenic changes in the haemagglutinin of Hong Kong influenza (H3N2) strains using a large panel of monoclonal antibodies.利用大量单克隆抗体对香港流感（H3N2）毒株血凝素中的抗原变化进行图谱绘制。
J Gen Virol. 1982 Sep;62 (Pt 1):153-69. doi: 10.1099/0022-1317-62-1-153.
5
Characterization of viruses from doves representing a new serotype of avian paramyxoviruses.代表禽副粘病毒新血清型的鸽子病毒特性分析。
Arch Virol. 1981;68(3-4):265-9. doi: 10.1007/BF01314580.
6
Classification of avian paramyxoviruses by immunodiffusion on the basis of antigenic specificity of their M protein antigens.
J Gen Virol. 1981 Jan;52(Pt 1):103-11. doi: 10.1099/0022-1317-52-1-103.
7
Determination of the number of nonoverlapping antigenic areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance.用单克隆抗体测定香港（H3N2）流感病毒血凝素上不重叠抗原区域的数量以及具有潜在流行病学意义的变异株的筛选
Virology. 1980 Jul 15;104(1):139-48. doi: 10.1016/0042-6822(80)90372-4.
8
Morphology of the isolated hemagglutinin and neuraminidase subunits of influenza virus.流感病毒分离的血凝素和神经氨酸酶亚基的形态学
Virology. 1969 May;38(1):105-19. doi: 10.1016/0042-6822(69)90132-9.
9
Antigenic hybrids of influenza A viruses with surface antigens to order.具有特定表面抗原的甲型流感病毒抗原杂种。
Virology. 1970 Nov;42(3):633-42. doi: 10.1016/0042-6822(70)90309-0.
10
Isolation and purification of the envelope proteins of Newcastle disease virus.新城疫病毒包膜蛋白的分离与纯化。
J Virol. 1973 Feb;11(2):263-71. doi: 10.1128/JVI.11.2.263-271.1973.