Babkina G T, Bausk E V, Graifer D M, Karpova G G, Matasova N B
FEBS Lett. 1984 May 21;170(2):290-4. doi: 10.1016/0014-5793(84)81330-7.
Photoreactive derivatives of E. coli tRNAPhe bearing arylazido groups on guanine residues (azido-tRNA) were used for affinity labelling of E. coli ribosomes in the region of the P-site when the A-site was either free or occupied by aminoacyl- or peptidyl-tRNA. Corresponding complexes of azido-tRNA with ribosomes and poly(U) were obtained both nonenzymatically and with the use of elongation factors. UV-irradiation of the complexes resulted in labelling of ribosomal proteins (preferentially of 30 S subunit). Proteins S9 and S21 were labelled only when the A-site was free; S14 - only when it was occupied; S11, S13, S19 - in both cases; S5, S7, S12, S20 - in some states.
在鸟嘌呤残基上带有芳基叠氮基团的大肠杆菌苯丙氨酸转运核糖核酸（azido - tRNA）的光反应性衍生物，用于在A位点自由或被氨酰基 - tRNA或肽基 - tRNA占据时，对大肠杆菌核糖体P位点区域进行亲和标记。azido - tRNA与核糖体和聚（U）的相应复合物既可以通过非酶方式获得，也可以利用延伸因子获得。对这些复合物进行紫外线照射会导致核糖体蛋白（优先标记30 S亚基）被标记。只有当A位点自由时，蛋白质S9和S21才会被标记；只有当A位点被占据时，S14才会被标记；在两种情况下，S11、S13、S19都会被标记；在某些状态下，S5、S7、S12、S20会被标记。
