Zhao Engui, Chen Yilong, Wang Hong, Chen Sijie, Lam Jacky W Y, Leung Chris W T, Hong Yuning, Tang Ben Zhong
†HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park, Nanshan, Shenzhen 518057, China.
‡Department of Chemistry, Division of Life Science, State Key Laboratory of Molecular Neuroscience, Institute for Advanced Study, Institute of Molecular Functional Materials, Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
ACS Appl Mater Interfaces. 2015 Apr 8;7(13):7180-8. doi: 10.1021/am509142k. Epub 2015 Mar 27.
The rapid acquisition of antibiotic resistance poses difficulties in the development of effective methods to eliminate pathogenic bacteria. New bactericides, especially those do not induce the emergence of resistance, are thus in great demand. In this work, we report an aggregation-induced emission fluorogen, TPE-Bac, for bacterial imaging and elimination. TPE-Bac can be readily dissolved in aqueous solution with weak emission. The presence of bacteria can turn on its emission, and thus no washing step is required in the imaging process. Meanwhile, TPE-Bac can be applied as a bactericide for elimination of bacteria. The amphiphilic TPE-Bac bearing two long alkyl chains and two positively charged amines can intercalate into the membrane of bacteria, increase membrane permeability and lead to dark toxicity. The efficiency of bacteria killing is greatly enhanced under light irradiation. TPE-Bac can serve as a photosensitizer to induce reactive oxygen species (ROS) generation, which ensures the efficient killing of bacteria. The TPE-Bac-containing agar plates can be continuously used for bacteria killing by applying light to induce ROS generation.
抗生素耐药性的快速产生给开发有效消除病原菌的方法带来了困难。因此，对新型杀菌剂，尤其是那些不会诱导耐药性产生的杀菌剂有巨大需求。在这项工作中，我们报道了一种用于细菌成像和消除的聚集诱导发光荧光团TPE-Bac。TPE-Bac能很容易地溶解于水溶液中，发射较弱。细菌的存在能开启其发光，因此成像过程中无需洗涤步骤。同时，TPE-Bac可作为杀菌剂用于消除细菌。带有两条长烷基链和两个带正电荷胺的两亲性TPE-Bac能插入细菌膜中，增加膜通透性并导致暗毒性。在光照下细菌杀灭效率大大提高。TPE-Bac可作为光敏剂诱导活性氧（ROS）生成，从而确保高效杀灭细菌。含TPE-Bac的琼脂平板可通过光照诱导ROS生成而持续用于杀灭细菌。
