Abegão Karina Gomes Barros, Bracale Bruno Nascimento, Delfim Inara Guastini, Santos Eliane Szücs dos, Laposy Cecília Braga, Nai Gisele Alborghetti, Giuffrida Rogério, Nogueira Rosa Maria Barilli
Oeste Paulista University, Presidente Prudente, SP, Brazil.
School of Veterinary Medicine, UNOESTE, Presidente Prudente, SP, Brazil.
Acta Cir Bras. 2015 Mar;30(3):209-15. doi: 10.1590/S0102-865020150030000008.
To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits.
Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel. Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17th day.
With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group.
Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.
评估异体富血小板血浆（PRP）凝胶对兔手术皮肤伤口愈合的作用。
采集一只健康犬的血液制备PRP，并向PRP中添加葡萄糖酸钙以形成凝胶。在6只兔的背部中线两侧各做一个手术切口，一侧随机分配用于局部涂抹生理溶液，另一侧分配用于异体PRP凝胶治疗。术后0、3、7、10、14和17天进行临床评估（体重、疼痛敏感性、肤色、水肿、充血、渗出、结痂和肉芽组织）和形态学评估。术后第17天进行组织学分析。
除第10天时出现结痂外，各实验组的临床变量无显著差异。在对照组和PRP治疗组中，比较各时间点伤口面积减少情况时均发现有差异。组织学结果表明，对照组和PRP治疗组之间无显著差异。
异体富血小板血浆凝胶可促进兔皮肤伤口愈合，且无不良反应。
