Meira Raquel de Oliveira, Braga Daniel Nogueira Mendes, Pinheiro Leni Safira Gonçalves, Amorim Izabela Ferreira Gontijo, Vasconcellos Leonardo de Souza, Alberti Luiz Ronaldo
Fellow PhD degree, Graduate Program in Pathology, Universidade Federal de Minas Gerais (UFMG), and Instituto de Ensino e Pesquisa (IEP), Santa Casa de Belo Horizonte, Brazil. Conception and design of the study; acquisition, analysis and interpretation of data; manuscript preparation and writing.
Graduate student, Department of Surgery, Medical School, UFMG, Belo Horizonte-MG, Brazil. Technical procedures, acquisition of data, manuscript preparation and writing.
Acta Cir Bras. 2020 Nov 23;35(10):e202001006. doi: 10.1590/s0102-865020200100000006. eCollection 2020.
To evaluate and compare the effects of homologous and heterologous PRP (Platelet-Rich Plasma) on the quality and speed of skin wound healing, compared to Poor Platelet Plasma (PPP).
Twenty-one male adult rabbits were used; two for preparing homologous PRP, with the rest of them separated randomly in three groups, according to the treatment received: PPP - control (n=5), homologous PRP (n=7), heterologous (n=7). Excisional skin wounds were made on the back of the animals, for the application of homologous and heterologous PPP and PRP. At the 14th post-operative day (POD), the animals were subjected to a new wound, and the treatments were inverted. The wounds were evaluated macroscopically and histologically.
A larger percentage of scar retraction was observed on the group treated with heterologous PRP, compared to homologous PRP, at the third POD, an increase of 25.03% (p=0.01). No other statistically significant differences among treatments were observed. Among every group, skin healing was efficient, without local adverse effects.
Heterologous PRP contributed with more tissue retraction at the beginning of the wound healing process. After this, there were no differences on the wound healing skin process treated with PRP or PPP. However, our findings suggest the presence of others plasmatic factors, besides platelets, which could also contribute to the wound healing process, and thus, should be further investigated.
与贫血小板血浆（PPP）相比，评估并比较同源和异源富血小板血浆（PRP）对皮肤伤口愈合质量和速度的影响。
使用21只成年雄性兔子；2只用于制备同源PRP，其余兔子根据接受的治疗随机分为三组：PPP-对照组（n=5）、同源PRP组（n=7）、异源PRP组（n=7）。在动物背部制造切除性皮肤伤口，用于涂抹同源和异源PPP及PRP。术后第14天（POD），给动物制造新的伤口，并颠倒治疗方式。对伤口进行宏观和组织学评估。
在术后第3天，与同源PRP组相比，异源PRP治疗组观察到更大比例的瘢痕收缩，增加了25.03%（p=0.01）。未观察到其他治疗组间有统计学意义的差异。在每组中，皮肤愈合有效，无局部不良反应。
异源PRP在伤口愈合过程开始时促进了更多的组织收缩。在此之后，用PRP或PPP治疗的伤口愈合皮肤过程没有差异。然而，我们的研究结果表明，除了血小板外，还存在其他血浆因子，它们也可能有助于伤口愈合过程，因此，应进一步研究。
