Danscher G, Møller-Madsen B
J Histochem Cytochem. 1985 Mar;33(3):219-28. doi: 10.1177/33.3.2579122.
A method for light and electron microscopic demonstration of mercury sulfides and mercury selenides in mammalian tissue is presented. Silver ions adhering to the surface of submicroscopic traces of mercury sulfides or selenides in the tissue are reduced to metallic silver by hydroquinone. Physical development thereupon renders deposits of mercury sulfides or mercury selenide visible as spheres of solid silver. Examples of localization of mercury in the central nervous system and various organs from animals exposed to mercury chloride or methyl mercury chloride with or without additional sodium selenide treatment are presented. Selenium treatment results in a considerable increase in the amount of mercury that can be made visible by silver amplification. After mercury chloride treatment, most of the mercury is localized in lysosomes and is only rarely seen in secretory granules. After simultaneous selenium treatment, mercury is also found in nuclei of proximal tubule cells in the kidney and in macrophages. The "sulfide-osmium" method for ultrastructural localization of mercury suggested by Silberberg, Lawrence, and Leider (Arch Environ Health 19:7, 1969) and the light microscopic method using a photographic emulsion suggested by Umeda, Saito, and Saito (Jpn J Exp Med 39:17, 1969) have been experimentally analyzed and commented on.
本文介绍了一种在哺乳动物组织中对硫化汞和硒化汞进行光镜和电镜示踪的方法。组织中亚微观痕迹的硫化汞或硒化汞表面附着的银离子通过对苯二酚还原为金属银。随后的物理显影使硫化汞或硒化汞沉积物呈现为固态银球而可见。文中展示了暴露于氯化汞或甲基氯化汞（有无额外硒化钠处理）的动物中枢神经系统和各种器官中汞的定位实例。硒处理导致通过银放大可见的汞量显著增加。氯化汞处理后，大部分汞定位于溶酶体，在分泌颗粒中很少见。同时进行硒处理后，在肾脏近端小管细胞的细胞核和巨噬细胞中也发现了汞。Silberberg、Lawrence和Leider（《环境卫生档案》19:7，1969年）提出的用于汞超微结构定位的“硫化物-锇”方法以及Umeda、Saito和Saito（《日本实验医学杂志》39:17，1969年）提出的使用照相乳剂的光镜方法已进行了实验分析和评论。
