Nga Nguyen Kim, Hoai Tran Thanh, Viet Pham Hung
School of Chemical Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet Road, Hanoi, Viet Nam.
School of Chemical Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet Road, Hanoi, Viet Nam.
Colloids Surf B Biointerfaces. 2015 Apr 1;128:506-514. doi: 10.1016/j.colsurfb.2015.03.001. Epub 2015 Mar 7.
This study presents a facile synthesis of biomimetic hydroxyapatite nanorod/poly(D,L) lactic acid (HAp/PDLLA) scaffolds with the use of solvent casting combined with a salt-leaching technique for bone-tissue engineering. Field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and energy-dispersive X-ray spectroscopy were used to observe the morphologies, pore structures of synthesized scaffolds, interactions between hydroxyapatite nanorods and poly(D,L) lactic acid, as well as the compositions of the scaffolds, respectively. Porosity of the scaffolds was determined using the liquid substitution method. Moreover, the apatite-forming capability of the scaffolds was evaluated through simulated body fluid (SBF) incubation tests, whereas the viability, attachment, and distribution of human osteoblast cells (MG 63 cell line) on the scaffolds were determined through alamarBlue assay and confocal laser microscopy after nuclear staining with 4',6-diamidino-2-phenylindole and actin filaments of a cytoskeleton with Oregon Green 488 phalloidin. Results showed that hydroxyapatite nanorod/poly(D,L) lactic acid scaffolds that mimic the structure of natural bone were successfully produced. These scaffolds possessed macropore networks with high porosity (80-84%) and mean pore sizes ranging 117-183 μm. These scaffolds demonstrated excellent apatite-forming capabilities. The rapid formation of bone-like apatites with flower-like morphology was observed after 7 days of incubation in SBFs. The scaffolds that had a high percentage (30 wt.%) of hydroxyapatite demonstrated better cell adhesion, proliferation, and distribution than those with low percentages of hydroxyapatite as the days of culture increased. This work presented an efficient route for developing biomimetic composite scaffolds, which have potential applications in bone-tissue engineering.
本研究介绍了一种简便的合成仿生羟基磷灰石纳米棒/聚（D，L）乳酸（HAp/PDLLA）支架的方法，该方法采用溶剂浇铸结合盐析技术用于骨组织工程。利用场发射扫描电子显微镜、傅里叶变换红外光谱和能量色散X射线光谱分别观察合成支架的形态、孔结构、羟基磷灰石纳米棒与聚（D，L）乳酸之间的相互作用以及支架的组成。使用液体置换法测定支架的孔隙率。此外，通过模拟体液（SBF）孵育试验评估支架的磷灰石形成能力，而在用4'，6-二脒基-2-苯基吲哚进行核染色并用俄勒冈绿488鬼笔环肽对细胞骨架的肌动蛋白丝进行染色后，通过alamarBlue测定法和共聚焦激光显微镜确定人成骨细胞（MG 63细胞系）在支架上的活力、附着和分布。结果表明，成功制备了模仿天然骨结构的羟基磷灰石纳米棒/聚（D，L）乳酸支架。这些支架具有高孔隙率（80-84%）的大孔网络，平均孔径在117-183μm范围内。这些支架表现出优异的磷灰石形成能力。在SBF中孵育7天后，观察到具有花状形态的类骨磷灰石的快速形成。随着培养天数的增加，羟基磷灰石含量高（30 wt.%）的支架比羟基磷灰石含量低的支架表现出更好的细胞粘附、增殖和分布。这项工作为开发仿生复合支架提供了一条有效途径，该支架在骨组织工程中具有潜在应用。
