Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain; CIC microGUNE, Arrasate, Mondragón, Spain.
CIC microGUNE, Arrasate, Mondragón, Spain.
Biosens Bioelectron. 2015 Aug 15;70:34-41. doi: 10.1016/j.bios.2015.03.017. Epub 2015 Mar 10.
A highly sensitive amperometric magnetoimmunosensor for the determination of ErbB2 protein, a well-known biomarker related to high-impact high-incidence diseases such as breast cancer, is described. A sandwich format involving the covalent immobilization of a specific capture antibody onto magnetic beads (MBs) and incubation of the modified MBs with a mixture solution of the antigen and a HRP-labeled detector antibody was used. The resulting modified MBs were captured on the surface of a disposable screen-printed carbon electrode (SPCE) and the amperometric responses at -0.20 V were measured. This ErbB2 magnetoimmunosensor exhibited a very low detection limit of 26 pg mL(-1) far below the established cut-off for this biomarker (15 ng mL(-1)) and was successfully applied to the quantitation of ErbB2 in human serum and cell lysates samples without any matrix effect. In addition, the developed assay allowed the assessment of ErbB2 status directly in intact breast cancer cells. The results correlated well with those obtained with a commercial ELISA method, thus demonstrating that the new magnetoimmunosensing platform offers a truthful and useful analytical tool to be easily applied in breast cancer diagnosis through either ErbB2 protein determination or breast cancer cell status detection.
本文描述了一种用于测定 ErbB2 蛋白的高灵敏度电流型磁免疫传感器。ErbB2 蛋白是一种与乳腺癌等高发高发性疾病密切相关的著名生物标志物。该传感器采用三明治结构，将特定的捕获抗体共价固定在磁性珠（MBs）上，并将修饰后的 MBs 与抗原和 HRP 标记的检测抗体的混合物孵育。所得修饰后的 MBs 被捕获在一次性丝网印刷碳电极（SPCE）的表面上，并在-0.20 V 下测量电流响应。该 ErbB2 磁免疫传感器的检测限非常低，为 26 pg mL(-1)，远低于该生物标志物的设定截止值（15 ng mL(-1)），并且可以成功应用于人血清和细胞裂解物样品中 ErbB2 的定量分析，而没有任何基质效应。此外，该方法还可以直接在完整的乳腺癌细胞中评估 ErbB2 状态。与商业 ELISA 方法的结果相关性良好，这表明新的磁免疫传感平台提供了一种真实有用的分析工具，可通过 ErbB2 蛋白测定或乳腺癌细胞状态检测，轻松应用于乳腺癌诊断。
