Maliogka Varvara I, Olmos Antonio, Pappi Polyxeni G, Lotos Leonidas, Efthimiou Konstantinos, Grammatikaki Garyfalia, Candresse Thierry, Katis Nikolaos I, Avgelis Apostolos D
Lab of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, 54124 Thessaloniki, Greece.
Instituto Valenciano de Investigaciones Agrarias (IVIA), Plant Protection and Biotechnology Center, 46113 Moncada, Valencia, Spain.
Virus Res. 2015 May 4;203:47-55. doi: 10.1016/j.virusres.2015.03.003. Epub 2015 Mar 16.
Roditis leaf discoloration (RLD), a graft-transmissible disease of grapevine, was first reported in Greece in the 1980s. Even though various native grapevine viruses were identified in the affected vines, the etiology of the disease remained unknown. In the present study, we used an NGS platform for sequencing siRNAs from a twenty-year old Roditis vine showing typical RLD symptoms. Analysis of the NGS data revealed the presence of various known grapevine viruses and viroids as well as a hitherto uncharacterized DNA virus. The circular genome of the new virus was fully reassembled. It is 6988 nts long and includes 4 open reading frames (ORFs). ORF1, ORF2 and ORF4 code for proteins with unknown functions while ORF3 encodes a polyprotein with motifs related to the replication, encapsidation and movement of the virus. Phylogenetic analysis classified the novel virus within the genus Badnavirus, with closest relationship to Fig badnavirus 1. Further studies showed that the new badnavirus is closely related with the RLD disease and the provisional name grapevine Roditis leaf discoloration-associated virus (GRLDaV) is proposed. Our findings extend the number of DNA viruses identified in grapevine, further drawing attention to the potential importance of this virus group on grapevine pathology.
罗迪蒂斯叶变色病（RLD）是葡萄的一种可通过嫁接传播的病害，于20世纪80年代在希腊首次报道。尽管在受影响的葡萄藤中鉴定出了多种本地葡萄病毒，但该病的病因仍然不明。在本研究中，我们使用了一个二代测序平台对一株表现出典型RLD症状的20年生罗迪蒂斯葡萄藤的小干扰RNA（siRNA）进行测序。对二代测序数据的分析揭示了多种已知葡萄病毒和类病毒的存在，以及一种迄今未鉴定的DNA病毒。新病毒的环状基因组已完全拼接完成。它长6988个核苷酸，包含4个开放阅读框（ORF）。ORF1、ORF2和ORF4编码功能未知的蛋白质，而ORF3编码一种多聚蛋白，其基序与病毒的复制、衣壳化和移动有关。系统发育分析将这种新型病毒归类于杆状DNA病毒属，与无花果杆状DNA病毒1关系最为密切。进一步的研究表明，这种新的杆状DNA病毒与RLD病密切相关，并提出了临时名称葡萄罗迪蒂斯叶变色相关病毒（GRLDaV）。我们的研究结果增加了在葡萄中鉴定出的DNA病毒的数量，进一步凸显了该病毒组在葡萄病理学上的潜在重要性。
