Chiumenti Michela, Giampetruzzi Annalisa, Morelli Massimiliano, Savino Vito Nicola, Martelli Giovanni Paolo, La Notte Pierfederico, Palmisano Francesco, Saldarelli Pasquale
CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, Via Amendola 122/D, 70126, Bari, Italy.
Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti (DiSSPA), Università degli Studi di Bari Aldo Moro, 70126, Bari, Italy.
Virus Genes. 2016 Jun;52(3):428-31. doi: 10.1007/s11262-016-1305-2. Epub 2016 Feb 29.
The complete nucleotide sequence and genome organization of a new Badnavirus isolated from the autochthonous grapevine variety "Bombino nero" from Apulia (Italy) was determined. The genome of this virus consists of 7097 nt and has four open reading frames (ORFs). Analysis of putative proteins encoded by each ORF revealed greatest sequence similarity to Grapevine Roditis leaf discoloration-associated virus w4 (GRLDaV; NC_027131). In a pairwise alignment with GLRDaV w4 genome sequence, the "Bombino Nero" sequence was 109 nt longer with a major 57 nt insertion between positions 2405 and 2413. Furthermore, its putative ORF4 is located after the ORF3, while in the GLRDaV w4 sequence, the putative ORF4 completely overlapped ORF3. Nucleotide analysis classifies this new Badnavirus as a GLRDaV strain, which was named GRLDaV-BN. Multi-year field observations showed that the GLRDaV-BN-infected vine was symptomless.
测定了从意大利普利亚地区本土葡萄品种“黑博比诺”中分离出的一种新型杆状DNA病毒的完整核苷酸序列和基因组结构。该病毒的基因组由7097个核苷酸组成，有四个开放阅读框（ORF）。对每个ORF编码的推定蛋白进行分析，发现其与葡萄罗迪蒂斯叶变色相关病毒w4（GRLDaV；NC_027131）的序列相似性最高。在与GLRDaV w4基因组序列的两两比对中，“黑博比诺”序列长109个核苷酸，在2405和2413位之间有一个57个核苷酸的主要插入。此外，其推定的ORF4位于ORF3之后，而在GLRDaV w4序列中，推定的ORF4与ORF3完全重叠。核苷酸分析将这种新型杆状DNA病毒归类为GLRDaV的一个菌株，命名为GRLDaV-BN。多年的田间观察表明，感染GRLDaV-BN的葡萄藤没有症状。
