对一些克罗地亚本土葡萄品种的筛选发现了多种病毒，包括新的病毒。
Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones.
作者信息
Vončina Darko, Almeida Rodrigo P P
机构信息
Department of Plant Pathology, University of Zagreb Faculty of Agriculture, 10 000, Zagreb, Croatia.
Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA.
出版信息
Arch Virol. 2018 Aug;163(8):2239-2243. doi: 10.1007/s00705-018-3850-6. Epub 2018 Apr 21.
Next-generation sequencing of total RNA samples from four Croatian autochthonous grapevine varieties revealed the presence of a novel virus in two grapevine accessions. The complete genome sequence of a novel virus, tentatively named "grapevine badnavirus 1" (GBV-1), was reconstructed from a de novo-assembled contig. GBV-1 has a genome of 7,145 nucleotides containing three ORFs with sequence similarity to other badnaviruses. In addition, several other viruses and viroids, including grapevine virus G, grapevine virus K/D, grapevine virus T, grapevine Roditis leaf discoloration-associated virus, grapevine yellow speckle viroids 1 and 2, and hop stunt viroid were detected and identified for the first time in Croatian grapevines in the course of this study.
对来自四个克罗地亚本土葡萄品种的总RNA样本进行的下一代测序显示，在两个葡萄种质中存在一种新型病毒。从一个从头组装的重叠群中重建了一种新型病毒的完整基因组序列，该病毒暂命名为“葡萄杆状DNA病毒1”（GBV-1）。GBV-1基因组有7145个核苷酸，包含三个与其他杆状DNA病毒具有序列相似性的开放阅读框。此外，在本研究过程中，还首次在克罗地亚葡萄中检测并鉴定出了其他几种病毒和类病毒，包括葡萄病毒G、葡萄病毒K/D、葡萄病毒T、罗迪蒂斯葡萄叶变色相关病毒、葡萄黄斑类病毒1和2以及啤酒花矮化类病毒。
Zotero 插件