Watanabe Miho, Kojima Hisaya, Fukui Manabu
The Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido, Japan.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1902-1907. doi: 10.1099/ijs.0.000197. Epub 2015 Mar 19.
A novel sulfate-reducing bacterium, designated strain Pf12BT, was isolated from sediment of meromictic Lake Harutori in Japan. Cells were vibroid (1.0 × 3.0-4.0 μm), motile and Gram-stain-negative. For growth, the optimum pH was 7.0-7.5 and the optimum temperature was 42-45 °C. Strain Pf12BT used sulfate, thiosulfate and sulfite as electron acceptors. The G+C content of the genomic DNA was 55.4 mol%. Major cellular fatty acids were C16 : 0 and C18 : 0. The strain was desulfoviridin-positive. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the order Desulfovibrionales in the class Deltaproteobacteria. The closest relative was Desulfomicrobium baculatum DSM 4028T with which it shared 91 % 16S rRNA gene sequence similarity. On the basis of phylogenetic and phenotypic characterization, a novel species of a new genus belonging to the family Desulfomicrobiaceae is proposed, Desulfoplanes formicivorans gen. nov., sp. nov. The type strain of Desulfoplanes formicivorans is Pf12BT ( = NBRC 110391T = DSM 28890T).
从日本哈鲁托里湖半咸水湖沉积物中分离出一种新型硫酸盐还原菌，命名为Pf12BT菌株。细胞呈弧状（1.0×3.0 - 4.0μm），具运动性，革兰氏染色阴性。该菌生长的最适pH为7.0 - 7.5，最适温度为42 - 45℃。Pf12BT菌株利用硫酸盐、硫代硫酸盐和亚硫酸盐作为电子受体。基因组DNA的G+C含量为55.4 mol%。主要细胞脂肪酸为C16 : 0和C18 : 0。该菌株脱硫绿素呈阳性。基于16S rRNA基因的系统发育分析表明，该新菌株属于δ-变形菌纲脱硫弧菌目。与其亲缘关系最近的是杆状脱硫微菌DSM 4028T，16S rRNA基因序列相似性为91%。基于系统发育和表型特征，提出了脱硫微菌科一个新属的一个新物种，甲酸脱硫平面菌属新种，甲酸脱硫平面菌。甲酸脱硫平面菌的模式菌株为Pf12BT（= NBRC 110391T = DSM 28890T）。
