Souza Pamella Macedo de, Goulart Fátima Regina de Vasconcelos, Marques Joana Montezano, Bizzo Humberto Ribeiro, Blank Arie Fitzgerald, Groposo Claudia, Sousa Maíra Paula de, Vólaro Vanessa, Alviano Celuta Sales, Moreno Daniela Sales Alviano, Seldin Lucy
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.
Instituto de Ciências Biológicas, Universidade Federal do Pará, Pará 66075-900, Brazil.
Molecules. 2017 Apr 19;22(4):648. doi: 10.3390/molecules22040648.
Strategies for the control of sulfate-reducing bacteria (SRB) in the oil industry involve the use of high concentrations of biocides, but these may induce bacterial resistance and/or be harmful to public health and the environment. Essential oils (EO) produced by plants inhibit the growth of different microorganisms and are a possible alternative for controlling SRB. We aimed to characterize the bacterial community of produced water obtained from a Brazilian petroleum facility using molecular methods, as well as to evaluate the antimicrobial activity of EO from different plants and their major components against NCIMB 13491 and against SRB growth directly in the produced water. Denaturing gradient gel electrophoresis revealed the presence of the genera and , , and the SRB in our produced water samples. Sequencing of insert-containing clones confirmed the presence of sequences related to . EO obtained from , LA44 and , as well as citral, linalool, eugenol and geraniol, greatly inhibited (minimum inhibitory concentration (MIC) = 78 µg/mL) the growth of in a liquid medium. The same MIC was obtained directly in the produced water with EO from LA44 (containing 82% citral) and with pure citral. These findings may help to control detrimental bacteria in the oil industry.
石油工业中控制硫酸盐还原菌（SRB）的策略包括使用高浓度的杀菌剂，但这些杀菌剂可能会诱导细菌产生抗性，和/或对公众健康和环境有害。植物产生的精油（EO）可抑制不同微生物的生长，是控制SRB的一种可能替代方法。我们旨在使用分子方法对从巴西石油设施获得的采出水中的细菌群落进行表征，并评估来自不同植物的EO及其主要成分对NCIMB 13491以及直接对采出水中SRB生长的抗菌活性。变性梯度凝胶电泳显示我们的采出水样品中存在 属、 属、 属和SRB 。对含 插入片段的克隆进行测序证实存在与 相关的序列。从 、LA44和 中获得的EO，以及柠檬醛、芳樟醇、丁香酚和香叶醇，在液体培养基中极大地抑制了 （最小抑菌浓度（MIC）= 78 µg/mL）的生长。使用来自LA44（含82%柠檬醛）的EO和纯柠檬醛直接在采出水中获得了相同的MIC。这些发现可能有助于控制石油工业中的有害细菌。
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