胆囊黏膜中促炎细胞因子（IL-1α、IL-6和TNF-α）免疫组化表达分析：急性和慢性结石性胆囊炎的比较研究

Analysis of immunohistochemical expression of proinflammatory cytokines (IL-1α, IL-6, and TNF-α) in gallbladder mucosa: comparative study in acute and chronic calculous cholecystitis.

作者信息

Kasprzak A, Szmyt M, Malkowski W, Przybyszewska W, Helak-Łapaj C, Seraszek-Jaros A, Surdacka A, Małkowska-Lanzafame A, Kaczmarek E

机构信息

Chair and Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland.

出版信息

Folia Morphol (Warsz). 2015;74(1):65-72. doi: 10.5603/FM.2015.0011.

DOI:10.5603/FM.2015.0011

PMID:25792398

Abstract

BACKGROUND

Several studies have shown increased serum levels of proinflammatory cytokines (IL-1α, IL-6, and TNF-α) in patients with cholelithiasis. The local expression of the proteins involved in pathogenesis of the disease is poorly recognised.

MATERIALS AND METHODS

The authors examined immunohistochemically (IHC) the expression status of IL-1α, IL-6, and TNF-α in gallbladder mucosa of the patients with cholelithiasis as related to acute (ACC) and chronic (CCC) types of cholecystitis. Proinflammatory cytokines were quantitatively evaluated in gallbladder mucosa (epithelium and lamina propria) in ACC (n = 16) and CCC (n = 55) groups using modern spatial visualisation technique.

RESULTS

Quantitative analysis of IHC signals showed no significant differences in IL-1α and IL-6, and immunoexpression in patients with ACC and CCC. A significantly greater IHC expression of TNF-α was detected in CCC as compared with ACC group. In either of the patient groups immunoexpression of IL-1α and of TNF-α was significantly higher than that of IL-6. Immunoexpression of TNF-α was significantly higher than that of IL-1α only in CCC group. A positive correlation was disclosed between IHC expression of IL-1α and body mass index in CCC group. IHC expression of TNF-α correlated positively with expression of CD68 molecule (histiocytic marker), number of leukocytes in blood and higher grading of gallbladder wall in ACC group.

CONCLUSIONS

A more pronounced IHC expression of TNF-α and IL-1α than IL-6 in both types of cholecystitis may suggest the role of these cytokines in pathogenesis of cholelithiasis. IHC expression of TNF- α shows better correlation with clinical/laboratory data in acute cholecystitis, and its quantitative prevalence over the remaining cytokines points to the role of the TNF-α in maintenance of inflammation in the course of cholelithiasis.

摘要

背景

多项研究表明，胆石症患者血清促炎细胞因子（IL-1α、IL-6和TNF-α）水平升高。疾病发病机制中相关蛋白的局部表达情况鲜为人知。

材料与方法

作者采用免疫组织化学（IHC）方法，检测了胆石症患者胆囊黏膜中IL-1α、IL-6和TNF-α的表达状况，并与急性胆囊炎（ACC）和慢性胆囊炎（CCC）类型相关。使用现代空间可视化技术，对ACC组（n = 16）和CCC组（n = 55）的胆囊黏膜（上皮和固有层）中的促炎细胞因子进行定量评估。

结果

IHC信号的定量分析显示，ACC和CCC患者的IL-1α和IL-6无显著差异，免疫表达情况相似。与ACC组相比，CCC组中TNF-α的IHC表达明显更高。在任一患者组中，IL-1α和TNF-α的免疫表达均显著高于IL-6。仅在CCC组中，TNF-α的免疫表达显著高于IL-1α。在CCC组中，IL-1α的IHC表达与体重指数呈正相关。在ACC组中，TNF-α的IHC表达与CD68分子（组织细胞标志物）的表达、血液中白细胞数量以及胆囊壁更高分级呈正相关。