Unità di Protistologia-Zoologia, Dipartimento di Biologia, Pisa, Italy.
Proc Jpn Acad Ser B Phys Biol Sci. 2006 Dec;82(9):359-74. doi: 10.2183/pjab.82.359. Epub 2006 Feb 12.
In the present paper we redescribe the ciliate Chattonidium setense Villeneuve 1937 combining morphological observations (live, stained, scanning, and transmission electron microscope) with behavioral notes and molecular data. Ultrastructural analysis revealed remarkable similarities between Chattonidium and representative members of the class Heterotrichea in cortical structure and cytoplasmic organization. The most similar genus for these aspects appears to be Condylostoma. To verify this relatedness, 18S rRNA genes from Chattonidium and from one Condylostoma species were sequenced. Phylogenetic analysis indicates Chattonidium belongs to the class Heterotrichea defined according to the modern taxonomy, and confirms its relatedness with Condylostoma already hypothesized by Villeneuve-Brachon (1940). The presence of the aboral cavity complex, a unique feature never described in other ciliates, and its peculiar organization revealed by ultrastructural analysis fully justify, in our opinion, the maintenance of Chattonidium in the separate family Chattonidiidae, established by Villeneuve-Brachon in 1940.
在本文中，我们将重新描述纤毛 Chattonidium setense Villeneuve 1937，结合形态学观察（活体、染色、扫描和透射电子显微镜）、行为注释和分子数据。超微结构分析显示，Chattonidium 与代表异毛类的成员在皮质结构和细胞质组织方面具有显著的相似性。在这些方面，最相似的属似乎是 Condylostoma。为了验证这种亲缘关系，我们对 Chattonidium 和一种 Condylostoma 物种的 18S rRNA 基因进行了测序。系统发育分析表明，Chattonidium 属于根据现代分类学定义的异毛类，并且证实了它与 Villeneuve-Brachon（1940）假设的 Condylostoma 的亲缘关系。顶腔复合体的存在，这是从未在其他纤毛类中描述过的独特特征，以及超微结构分析揭示的其特殊组织，充分证明了我们认为，根据 Villeneuve-Brachon 于 1940 年建立的独立的 Chattonidiidae 家族，维持 Chattonidium 是合理的。
