Lee Hye-Lan, Oh Jinsoo, Yun Yeomin, Lee Hye Yeong, You Youngsang, Che Lihua, Lee Minhyung, Kim Keung Nyun, Ha Yoon
aDepartment of Neurosurgery, Spine & Spinal Cord Institute bBrain Korea 21 PLUS Project for Medical Science, College of Medicine, Yonsei University cDepartment of Bioengineering, College of Engineering, Hanyang University, Seoul, Korea.
Neuroreport. 2015 May 6;26(7):399-404. doi: 10.1097/WNR.0000000000000359.
Previously, we determined that vascular endothelial growth factor (VEGF) improves the survival of neural stem cells (NSCs) transplanted into an ischemic environment and effectively enhances angiogenesis. Here, we applied NSCs expressing VEGF (SV-VEGF-NSCs) to treat neuropathic pain. In this study, our goal was to verify the therapeutic effect of SV-VEGF-NSCs by transplanting the cells in a sciatic nerve injury model. We compared the amount of VEGF secreted from DsRed-NSCs (control) or SV-VEGF-NSCs and observed that SV-VEGF-NSCs have a much higher expression level of VEGF. We next investigated whether transplantation with SV-VEGF-NSCs aids functional recovery and pain reduction. We confirmed that transplantation with SV-VEGF-NSCs enhances functional recovery, pain reduction, and remyelination as well as the number of blood vessels compared with the control groups. Our results show that VEGF aids functional recovery and pain reduction in a sciatic nerve injury model.
此前，我们确定血管内皮生长因子（VEGF）可提高移植到缺血环境中的神经干细胞（NSCs）的存活率，并有效促进血管生成。在此，我们应用表达VEGF的神经干细胞（SV-VEGF-NSCs）来治疗神经性疼痛。在本研究中，我们的目标是通过将细胞移植到坐骨神经损伤模型中来验证SV-VEGF-NSCs的治疗效果。我们比较了DsRed-NSCs（对照）或SV-VEGF-NSCs分泌的VEGF量，观察到SV-VEGF-NSCs具有更高的VEGF表达水平。接下来，我们研究了移植SV-VEGF-NSCs是否有助于功能恢复和减轻疼痛。我们证实，与对照组相比，移植SV-VEGF-NSCs可增强功能恢复、减轻疼痛、促进髓鞘再生以及增加血管数量。我们的结果表明，VEGF有助于坐骨神经损伤模型中的功能恢复和减轻疼痛。
