Robinson H L, Miles B D
Virology. 1985 Feb;141(1):130-43. doi: 10.1016/0042-6822(85)90189-8.
DNAs from 19 cases of avian leukosis virus-induced osteopetrosis have been analyzed for viral sequences. Among these were instances of rapid, intermediate, and slow onset osteopetrosis. The DNAs from osteopetrotic bone contained no evidence for osteopetrosis being caused by proviral insertions into or viral transductions of a host protooncogene. Instead, DNAs from osteopetrotic bone displayed evidence for osteopetrosis being associated with the persistent synthesis of viral DNA. Each of the 19 DNAs contained unintegrated as well as integrated viral DNA. Rapid onset osteopetrosis contained about 3X more viral and proviral DNA than intermediate or late onset osteopetrosis. Unintegrated viral DNA could not be detected in DNAs extracted from the bursa bone marrow of osteopetrotic chickens or in DNA extracted from the normal bones of an avian leukosis virus-infected chicken. Thus, the persistent synthesis of unintegrated viral DNA was observed in osteopetrotic but not normal tissues of avian leukosis virus-infected chickens.
对19例禽白血病病毒诱导的骨质石化病例的DNA进行了病毒序列分析。其中包括快速、中度和缓慢发病的骨质石化病例。骨质石化骨的DNA没有证据表明骨质石化是由前病毒插入宿主原癌基因或病毒转导宿主原癌基因所致。相反，骨质石化骨的DNA显示出骨质石化与病毒DNA的持续合成有关。19个DNA样本均含有未整合以及整合的病毒DNA。快速发病的骨质石化所含的病毒和前病毒DNA比中度或晚期发病的骨质石化多约3倍。在从骨质石化鸡的法氏囊骨髓中提取的DNA或从感染禽白血病病毒的鸡的正常骨骼中提取的DNA中均未检测到未整合的病毒DNA。因此，在感染禽白血病病毒的鸡的骨质石化组织而非正常组织中观察到了未整合病毒DNA的持续合成。
