Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil.
Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil.
Biotechnol Adv. 2015 Sep-Oct;33(5):394-411. doi: 10.1016/j.biotechadv.2015.03.010. Epub 2015 Mar 18.
Biocatalysis currently is focusing on enzymatic and multi-enzymatic cascade processes instead of single steps imbedded into chemical pathways. Alongside this scientific revolution, this review provides an overview on multi-enzymatic cascades that are responsible for the biosynthesis of some terpenes, alkaloids and polyethers, which are important classes of natural products. Herein, we illustrate the development of studies inspired by multi- and chemo-enzymatic approaches to build the core moieties of polyethers, polypeptide alkaloids, piperidines and pyrrolidines promoted by the joint action of oxidoreductases, hydrolases, cyclases, transaminases and imine reductases.
生物催化目前专注于酶和多酶级联反应过程，而不是嵌入化学途径的单个步骤。在这场科学革命中，本篇综述概述了负责萜类、生物碱和聚醚等一些重要天然产物生物合成的多酶级联反应。文中举例说明了多酶和化学酶方法的发展，这些方法通过氧化还原酶、水解酶、环化酶、转氨酶和亚胺还原酶的共同作用，构建了聚醚、多肽生物碱、哌啶和吡咯烷的核心部分。
