Yang Rongchang, Brice Belinda, Ryan Una
School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia.
Exp Parasitol. 2015 Jun;153:123-8. doi: 10.1016/j.exppara.2015.03.005. Epub 2015 Mar 17.
Eimeria haematodi was first described in 1977 from the rainbow lorikeet (Trichoglossus haematodus) in Papua New Guinea. In the present study, we re-describe this coccidian species morphologically and molecularly from a rainbow lorikeet bird in Western Australia (WA). The oocysts were ovoid to slightly piriform and measured 28.5-37.8 by 25.8-33.0 µm (33.3 by 28.1 µm). Oocyst wall was approximately 1.5 µm thick and bilayered. Micropyle (5-7 µm) and oocyst residuum (8.0-10.0 µm) present; polar granule was absent. Sporocysts ellipsoidal, 11.8-13.6 by 8.0-9.6 µm (12.2 by 8.3 µm), with thin convex Stieda body and granular sporocyst residuum (4.0-5.0 µm). Molecular characterization of E. haematodi was conducted at 18S ribosomal RNA and the mitochondrial cytochrome oxidase gene (COI) loci. At the 18S ribosomal RNA locus, E. haematodi shared 98.1% genetic similarity to E. alabamensis from cattle in New South Wales, Australia. At COI locus, E. haematodi was closest (92.3% similarity) to E. praecox from domestic chickens (Gallus gallus domesticus) from Canada and China.
血变艾美耳球虫于1977年首次在巴布亚新几内亚的虹彩吸蜜鹦鹉（Trichoglossus haematodus）中被描述。在本研究中，我们从西澳大利亚州（WA）的一只虹彩吸蜜鹦鹉鸟身上，对这种球虫进行了形态学和分子学的重新描述。卵囊呈卵圆形至略梨形，大小为28.5 - 37.8×25.8 - 33.0μm（33.3×28.1μm）。卵囊壁约1.5μm厚，双层。有微孔（5 - 7μm）和卵囊残体（8.0 - 10.0μm）；无极粒。孢子囊椭圆形，11.8 - 13.6×8.0 - 9.6μm（12.2×8.3μm），有薄的凸形斯氏体和颗粒状孢子囊残体（4.0 - 5.0μm）。对血变艾美耳球虫进行了18S核糖体RNA和线粒体细胞色素氧化酶基因（COI）位点的分子特征分析。在18S核糖体RNA位点，血变艾美耳球虫与来自澳大利亚新南威尔士州牛的阿拉巴马艾美耳球虫有98.1%的遗传相似性。在COI位点，血变艾美耳球虫与来自加拿大和中国的家鸡（Gallus gallus domesticus）的早熟艾美耳球虫最为接近（相似性为92.3%）。
