Yang Rongchang, Brice Belinda, Ryan Una
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia.
Int J Parasitol Parasites Wildl. 2016 Jan 12;5(1):34-9. doi: 10.1016/j.ijppaw.2016.01.003. eCollection 2016 Apr.
A new Eimeria species is described from a red-capped parrot (Purpureicephalus spurius). Sporulated oocysts (n = 31) were spherical to subspherical, with a rough bilayered oocyst wall 0.8 μm thick. Oocysts measured 24.0 × 22.8 (20.4-26.4 × 18.3-25.9) μm, oocyst length/width ratio, 1.10. Oocyst residuum, polar granule and micropyle were absent. Sporocysts are elongate-ovoid, 11.0 × 7.3 (12.7-9.2 × 7.9-6.6) μm, sporocyst length/width ratio, 1.51 (1.33-1.71). The thin convex Stieda body and indistinct substieda bodies were present and the sporocyst residuum was composed of numerous small granules less than 1.0 μm in diameter dispersed randomly. Each sporocyst contained 2 sausage-shaped sporozoites in head-to-tail arrangement. The sporozoite nuclei were located centrally surrounded by refractile bodies. Molecular analysis was conducted at two loci; the 18S ribosomal RNA gene and the cytochrome c oxidase subunit I gene. At the18S locus, the new isolate shared 99.0% genetic similarity with Eimeria dispersa and Eimeria innocua from the turkey. At the cytochrome c oxidase subunit I gene locus, this new isolate was most closely related to E. dispersa and E. innocua, presented 99.0% and 98.0% genetic similarity, respectively. This new isolate and E. dispersa grouped together in the same clade. Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria purpureicephali n. sp. after its host, the red-capped parrot (Purpureicephalus spurius).
在一只红顶鹦鹉（Purpureicephalus spurius）体内发现了一种新的艾美耳球虫。孢子化卵囊（n = 31）呈球形至亚球形，卵囊壁粗糙，双层结构，厚0.8μm。卵囊大小为24.0×22.8（20.4 - 26.4×18.3 - 25.9）μm，卵囊长宽比为1.10。无卵囊残体、极粒和微孔。孢子囊呈长椭圆形，11.0×7.3（12.7 - 9.2×7.9 - 6.6）μm，孢子囊长宽比为1.51（1.33 - 1.71）。有薄的凸形斯氏体和不明显的亚斯氏体，孢子囊残体由许多直径小于1.0μm的小颗粒随机分散组成。每个孢子囊内含有2个腊肠形子孢子，呈头尾排列。子孢子核位于中央，周围有折光体。在两个基因位点进行了分子分析，即18S核糖体RNA基因和细胞色素c氧化酶亚基I基因。在18S位点，新分离株与来自火鸡的分散艾美耳球虫和无害艾美耳球虫的遗传相似性为99.%. 在细胞色素c氧化酶亚基I基因位点，该新分离株与分散艾美耳球虫和无害艾美耳球虫关系最为密切，遗传相似性分别为99.0%和98.0%。该新分离株与分散艾美耳球虫聚在同一进化枝中。基于形态学和分子数据，该分离株是一种新的球虫寄生虫物种，以其宿主红顶鹦鹉（Purpureicephalus spurius）命名为紫头艾美耳球虫（Eimeria purpureicephali n. sp.） 。
