Wesselink Monique, Bergwerff Leonie, Hoogmoed Daniëlle, Kloosterman Ate D, Kuiper Irene
Netherlands Forensic Institute, P.O. Box 24044, 2490 AA The Hague, The Netherlands; Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 94248, 1090 GE Amsterdam, The Netherlands.
Netherlands Forensic Institute, P.O. Box 24044, 2490 AA The Hague, The Netherlands; Institute of Biology Leiden, Leiden University, P.O. Box 9505, 2300 RA Leiden, The Netherlands.
Forensic Sci Int Genet. 2015 Jul;17:25-32. doi: 10.1016/j.fsigen.2015.03.004. Epub 2015 Mar 12.
Different portions of the feline mitochondrial DNA control region (CR) were evaluated for their informative value in forensic investigations. The 402bp region located between RS2 and RS3 described most extensively in the past is not efficient for distinguishing between the majority of Dutch cats, illustrated by a random match probability (RMP) of 41%. Typing of the whole region between RS2 and RS3, and additional typing of the 5'portion of the feline CR decreases the RMP to 29%, increasing the applicability of such analyses for forensic investigations. The haplotype distribution in Dutch random bred cats (N=113) differs greatly from the distributions reported for other countries, with a single haplotype NL-A1 present in 54% of the population. The three investigated breeds showed haplotype distributions differing from each other and the random bred cats with haplotype NL-A1 accounting for 4%, 29% and 32% of Maine Coon, Norwegian forest cats and Siamese & Oriental cats. These results indicate the necessity of validating haplotype frequencies within continents and regions prior to reporting the value a mtDNA match. In cases where known purebred cats are involved, further investigation of the breed may be valuable.
对猫线粒体DNA控制区（CR）的不同部分在法医调查中的信息价值进行了评估。过去描述最广泛的位于RS2和RS3之间的402bp区域，对于区分大多数荷兰猫并不有效，随机匹配概率（RMP）为41%就说明了这一点。对RS2和RS3之间的整个区域进行分型，并对猫CR的5'部分进行额外分型，可将RMP降至29%，提高了此类分析在法医调查中的适用性。荷兰随机繁殖猫（N = 113）的单倍型分布与其他国家报告的分布有很大差异，54%的种群中存在单一单倍型NL - A1。所研究的三个品种的单倍型分布彼此不同，并且随机繁殖猫中，单倍型NL - A1在缅因猫、挪威森林猫以及暹罗猫和东方猫中分别占4%、29%和32%。这些结果表明，在报告线粒体DNA匹配的价值之前，有必要在各大洲和地区内验证单倍型频率。在涉及已知纯种猫的案件中，对品种的进一步调查可能是有价值的。
