Głażewska Iwona, Kijewski Tomasz
Department of Plant Taxonomy and Nature Conservation, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
Department of Genetics and Marine Biotechnology, Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712, Sopot, Poland.
Forensic Sci Int Genet. 2017 Mar;27:116-122. doi: 10.1016/j.fsigen.2016.12.010. Epub 2016 Dec 29.
Domestic cats from Eastern Europe have been poorly represented in studies on mitochondrial DNA diversity for forensic purposes until now. The aim of the present study was to contribute to closing this gap. The genetic structure and the origin of a cat population in Poland were examined against the background of human migrations over the centuries. One hundred and eighty-one cats from animal shelters in seven cities were genotyped. Twenty-one mtDNA haplotypes were found, with only one haplotype present in each of the populations, at an average frequency of 63.54%, and 13 haplotypes being found only in single populations. The analysis revealed the unexpectedly high frequency of haplotype PL02, in previous studies observed only in single cats. Differences in the number of the haplotypes, from four to eight, were observed among the shelters. The findings are discussed with regard to a world-wide database of feline sequences and to the complicated history of Poland. The study underscores the necessity of creating local databases of haplotypes that are of high evidentiary value to the forensic investigations conducted in a given country.
直到现在，东欧家猫在用于法医目的的线粒体DNA多样性研究中一直未得到充分体现。本研究的目的是为填补这一空白做出贡献。在几个世纪以来人类迁徙的背景下，研究了波兰猫种群的遗传结构和起源。对来自七个城市动物收容所的181只猫进行了基因分型。共发现21种线粒体DNA单倍型，每个种群中仅存在一种单倍型，平均频率为63.54%，13种单倍型仅在单个种群中发现。分析发现单倍型PL02的频率出乎意料地高，在之前的研究中仅在单只猫中观察到。在不同收容所中观察到单倍型数量的差异，从四种到八种不等。结合全球猫序列数据库和波兰复杂的历史对研究结果进行了讨论。该研究强调了创建具有高证据价值的本地单倍型数据库对于在特定国家进行法医调查的必要性。
