Ottolini Barbara, Lall Gurdeep Matharu, Sacchini Federico, Jobling Mark A, Wetton Jon H
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
IDEXX Laboratories Ltd., Grange House, Sandbeck Way, Wetherby, West Yorkshire, LS22 7DN, UK.
Forensic Sci Int Genet. 2017 Mar;27:149-155. doi: 10.1016/j.fsigen.2016.12.008. Epub 2016 Dec 21.
DNA variation in 402bp of the mitochondrial control region flanked by repeat sequences RS2 and RS3 was evaluated by Sanger sequencing in 152 English domestic cats, in order to determine the significance of matching DNA sequences between hairs found with a victim's body and the suspect's pet cat. Whilst 95% of English cats possessed one of the twelve globally widespread mitotypes, four new variants were observed, the most common of which (2% frequency) was shared with the evidential samples. No significant difference in mitotype frequency was seen between 32 individuals from the locality of the crime and 120 additional cats from the rest of England, suggesting a lack of local population structure. However, significant differences were observed in comparison with frequencies in other countries, including the closely neighbouring Netherlands, highlighting the importance of appropriate genetic databases when determining the evidential significance of mitochondrial DNA evidence.
为了确定在受害者尸体上发现的毛发与嫌疑人宠物猫之间DNA序列匹配的重要性，通过桑格测序法对152只英国家猫线粒体控制区402bp中位于重复序列RS2和RS3两侧的DNA变异进行了评估。虽然95%的英国猫拥有全球广泛分布的12种线粒体单倍型之一，但观察到了4种新的变异，其中最常见的（频率为2%）与证据样本相同。在来自犯罪地点的32只猫和来自英格兰其他地区的120只猫之间，线粒体单倍型频率没有显著差异，这表明缺乏当地种群结构。然而，与其他国家（包括紧邻的荷兰）的频率相比，观察到了显著差异，这突出了在确定线粒体DNA证据的证据重要性时，合适的基因数据库的重要性。
