Silva Fernanda D F, Espinoza Luis R L, Tonietti Paloma O, Barbosa Bruna R P, Gregori Fabio
Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Av. Professor Dr. Orlando Marques de Paiva, 87, 05508-270 São Paulo, SP, Brazil.
Infect Genet Evol. 2015 Jun;32:239-54. doi: 10.1016/j.meegid.2015.03.016. Epub 2015 Mar 19.
Group A rotaviruses (RVAs) are leading causes of viral diarrhea in children and in the young of many animal species, particularly swine. In the current study, porcine RVAs were found in fecal specimens from symptomatic piglets on 4 farms in Brazil during the years of 2012-2013. Using RT-PCR, Sanger nucleotide sequencing, and phylogenetic analyses, the whole genomes of 12 Brazilian porcine RVA strains were analyzed. Specifically, the full-length open reading frame (ORF) sequences were determined for the NSP2-, NSP3-, and VP6-coding genes, and partial ORF sequences were determined for the VP1-, VP2-, VP3-, VP4-, VP7-, NSP1-, NSP4-, and NSP5/6-coding genes. The results indicate that all 12 strains had an overall porcine-RVA-like backbone with most segments being designated as genotype 1, with the exception of the VP6- and NSP1-coding genes, which were genotypes I5 and A8, respectively. These results add to our growing understanding of porcine RVA genetic diversity and will provide a platform for monitoring the role of animals as genetic reservoirs of emerging human RVAs strains.
A组轮状病毒（RVAs）是导致儿童以及许多动物幼崽（尤其是猪）病毒性腹泻的主要病因。在本研究中，于2012年至2013年期间在巴西4个农场有症状仔猪的粪便样本中发现了猪RVAs。利用逆转录聚合酶链反应（RT-PCR）、桑格核苷酸测序和系统发育分析，对12株巴西猪RVA毒株的全基因组进行了分析。具体而言，测定了NSP2、NSP3和VP6编码基因的全长开放阅读框（ORF）序列，并测定了VP1、VP2、VP3、VP4、VP7、NSP1、NSP4和NSP5/6编码基因的部分ORF序列。结果表明，所有12株毒株总体上具有类似猪RVA的主干结构，大多数片段被指定为基因型1，但VP6和NSP1编码基因除外，它们分别为I5和A8基因型。这些结果增进了我们对猪RVA遗传多样性的认识，并将为监测动物作为新兴人类RVA毒株基因库的作用提供一个平台。
