Romanov S V, Smolianitskiĭ A G, Molin Iu A
Sud Med Ekspert. 2014 May-Jun;57(3):25-8.
The objective of the present study was to elucidate the genetic profiles of the biological materials taken from four graves in the Demidov family vault in order to establish kinship between its members. According to the archival documents, two graves contained the remains of Pyotr Grigor'evich Demidov, an adjutant-general for the emperor Aleksandr II, and his wife Elizaveta Nikolaevna Demidova (Bezobrazova). Also, it was supposed that two other graves contained the remains of Grigory Petrovich Demidov and Ekaterina Petrovna Demidova (married name princess Kudasheva), the son and the daughter of P.G. Demidov and E.N. Demidova. The bodies remained in the half-ruined crypt during approximately 150 years under conditions of enhanced humidity and seasonal temperature fluctuations which made their bone tissue virtually unsuitable for the genetic analysis. Genotyping was performed with the use of standard AmpF/STR Identifiler-TM and AmpF/STR Yfiler-TM kits ("Applied Biosystems", USA). As a result of the study, the skeletal remains of the boy from grave No2 were identified as actually belonging to the son of P.G. Demidov and E.N. Demidova with a probability of no less than 99.999999998%. whereas the girl buried in grave No4, was not the daughter of these parents.
本研究的目的是阐明从德米多夫家族墓穴中四座坟墓取出的生物材料的基因图谱，以确定其成员之间的亲属关系。根据档案文件，两座坟墓中埋葬着亚历山大二世皇帝的副官长彼得·格里戈里耶维奇·德米多夫及其妻子伊丽莎白·尼古拉耶夫娜·德米多娃（别佐布拉佐娃）的遗体。此外，据推测，另外两座坟墓中埋葬着格里戈里·彼得罗维奇·德米多夫和叶卡捷琳娜·彼得罗夫娜·德米多娃（婚后姓氏为库达舍娃公主）的遗体，他们是P.G.德米多夫和E.N.德米多娃的儿子和女儿。这些尸体在湿度较高和季节性温度波动的条件下，在半毁的地下室中存放了大约150年，这使得他们的骨组织几乎不适用于基因分析。使用标准的AmpF/STR Identifiler-TM和AmpF/STR Yfiler-TM试剂盒（美国“应用生物系统公司”）进行基因分型。研究结果表明，2号墓中男孩的骨骼遗骸被确定实际上属于P.G.德米多夫和E.N.德米多娃的儿子，概率不低于99.999999998%。而埋葬在4号墓中的女孩并非这对父母的女儿。
