Can T V, Ni Q Z, Griffin R G
Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.
J Magn Reson. 2015 Apr;253:23-35. doi: 10.1016/j.jmr.2015.02.005.
Dynamic nuclear polarization (DNP) is a technique used to enhance signal intensities in NMR experiments by transferring the high polarization of electrons to their surrounding nuclei. The past decade has witnessed a renaissance in the development of DNP, especially at high magnetic fields, and its application in several areas including biophysics, chemistry, structural biology and materials science. Recent technical and theoretical advances have expanded our understanding of established experiments: for example, the cross effect DNP in samples spinning at the magic angle. Furthermore, new experiments suggest that our understanding of the Overhauser effect and its applicability to insulating solids needs to be re-examined. In this article, we summarize important results of the past few years and provide quantum mechanical explanations underlying these results. We also discuss future directions of DNP and current limitations, including the problem of resolution in protein spectra recorded at 80-100 K.
动态核极化(DNP)是一种用于在核磁共振(NMR)实验中通过将电子的高极化转移到其周围原子核来增强信号强度的技术。在过去十年中,DNP的发展迎来了复兴,特别是在高磁场下,并且它在生物物理学、化学、结构生物学和材料科学等多个领域得到了应用。最近的技术和理论进展扩展了我们对既定实验的理解:例如,在魔角旋转的样品中的交叉效应DNP。此外,新的实验表明,我们对奥弗豪泽效应及其在绝缘固体中的适用性的理解需要重新审视。在本文中,我们总结了过去几年的重要成果,并提供了这些结果背后的量子力学解释。我们还讨论了DNP的未来方向和当前的局限性,包括在80-100K下记录的蛋白质光谱中的分辨率问题。
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