‡Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-100, Republic of Korea.
§College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea.
J Agric Food Chem. 2015 Apr 8;63(13):3587-92. doi: 10.1021/acs.jafc.5b01027. Epub 2015 Mar 30.
Two new quinolinone alkaloids and 13 known compounds were isolated from chestnut (Castanea crenata Sieb) honey. Two new compounds were determined to be 3-dihydro-spiro[2(1H),3'(1'H)-diquinoline]-3',4,4'-trione (spirodiquinolinone) and 3-(2'-piperidine)-kynurenic acid. In addition, 2,3-dihydropyrrolo[1,2-a]quinazolin-5(1H)-one was identified for the first time from nature. In addition, 2,3-dihydropyrrolo[1,2-a]quinazolin-5(1H)-one was newly identified from chestnut honey, although this compound has been synthesized before. The structures were determined by the NMR and electrospray ionization-mass spectroscopy (ESI-MS). Three compounds were qualified and quantitated in chestnut honey by selective multiple reaction monitoring (MRM) detection of LC-ESI-MS using the isolated compounds as external standards.
从板栗（Castanea crenata Sieb）蜂蜜中分离得到两种新的喹啉酮生物碱和 13 种已知化合物。两种新化合物被确定为 3-二氢螺[2(1H)，3'(1'H)-二喹啉]-3'，4，4'-三酮（螺二喹啉酮）和 3-（2'-哌啶）-犬尿氨酸。此外，2,3-二氢吡咯并[1,2-a]喹唑啉-5(1H)-酮也是首次从自然界中鉴定出来的。此外，尽管之前已经合成过这种化合物，但它也首次从板栗蜂蜜中被新鉴定出来。这些结构是通过 NMR 和电喷雾电离质谱（ESI-MS）确定的。通过使用分离出的化合物作为外标，采用 LC-ESI-MS 的选择性多重反应监测（MRM）检测，在板栗蜂蜜中对三种化合物进行了定性和定量分析。
