Noh Jin Hyeong, Reddy Kondreddy Eswar, Choe Se Eun, Yoo Mi Sun, Doan Huong Thi Thanh, Kweon Chang Hee, Ramya Mummadireddy, Yoon Byoung-Su, Nguyen Lien Thi Kim, Nguyen Thuy Thi Dieu, Van Quyen Dong, Jung Suk-Chan, Chang Ki-Yoon, Kang Seung Won
Parasitology and Insect Disease Research Laboratory, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang 420-480, South Korea.
Virus Genes. 2013 Apr;46(2):362-8. doi: 10.1007/s11262-012-0859-x. Epub 2012 Dec 14.
The black queen cell virus (BQCV), a picorna-like honeybee virus, was first isolated from queen larvae and pupae of honeybees found dead in their cells. BQCV is the most common cause of death in queen larvae. Phylogenetic analysis of two Apis cerana and three Apis mellifera BQCV genotypes collected from honeybee colonies in different regions of South Korea, central European BQCV genotypes, and a South African BQCV reference genotype was performed on a partial helicase enzyme coding region (ORF1) and a partial structural polypeptide coding region (ORF2). The phylogeny based on the ORF2 region showed clustering of all the Korean genotypes corresponding to their geographic origin, with the exception of Korean Am str3 which showed more similarity to the central European and the South African reference genotype. However, the ORF1-based tree exhibited a different distribution of the Korean strains, in which A. cerana isolates formed one cluster and all A. mellifera isolates formed a separate cluster. The RT-PCR assay described in this study is a sensitive and reliable method for the detection and classification of BQCV strains from various regions of Korea. BQCV infection is present in both A. cerana and A. mellifera colonies. With this in mind, the present study examined the transmission of honeybee BQCV infections between A. cerana and A. mellifera.
黑王后细胞病毒（BQCV）是一种类似小核糖核酸病毒的蜜蜂病毒，最初是从死于巢房中的蜂王幼虫和蛹中分离出来的。BQCV是蜂王幼虫死亡的最常见原因。对从韩国不同地区的蜂群中收集的两种中华蜜蜂和三种西方蜜蜂BQCV基因型、中欧BQCV基因型以及一种南非BQCV参考基因型，在部分解旋酶编码区（ORF1）和部分结构多肽编码区（ORF2）上进行了系统发育分析。基于ORF2区域的系统发育显示，除了韩国西方蜜蜂str3与中欧和南非参考基因型更相似外，所有韩国基因型都根据其地理来源聚类。然而，基于ORF1的树状图显示韩国菌株的分布不同，其中中华蜜蜂分离株形成一个簇，所有西方蜜蜂分离株形成一个单独的簇。本研究中描述的RT-PCR检测方法是一种灵敏可靠的方法，用于检测和分类来自韩国不同地区的BQCV菌株。中华蜜蜂和西方蜜蜂蜂群中均存在BQCV感染。考虑到这一点，本研究检测了蜜蜂BQCV感染在中华蜜蜂和西方蜜蜂之间的传播情况。
