Faculty of Agriculture, Department of Plant Protection, University of Akdeniz, Antalya, Turkey.
Department of Plant Pathology, M.Y. Genetik Agriculture Technology Laboratory, Antalya, Turkey.
Theor Appl Genet. 2018 Oct;131(10):2099-2105. doi: 10.1007/s00122-018-3136-0. Epub 2018 Jul 7.
Modern plant breeding heavily relies on the use of molecular markers. In recent years, next generation sequencing (NGS) emerged as a powerful technology to discover DNA sequence polymorphisms and generate molecular markers very rapidly and cost effectively, accelerating the plant breeding programmes. A single dominant locus, Frl, in tomato provides resistance to the fungal pathogen Fusarium oxysporum f. sp. radicis-lycopersici (FORL), causative agent of Fusarium crown and root rot. In this study, we describe the generation of molecular markers associated with the Frl locus. An F mapping population between an FORL resistant and a susceptible cultivar was generated. NGS technology was then used to sequence the genomes of a susceptible and a resistant parent as well the genomes of bulked resistant and susceptible F lines. We zoomed into the Frl locus and mapped the locus to a 900 kb interval on chromosome 9. Polymorphic single-nucleotide polymorphisms (SNPs) within the interval were identified and markers co-segregating with the resistant phenotype were generated. Some of these markers were tested successfully with commercial tomato varieties indicating that they can be used for marker-assisted selection in large-scale breeding programmes.
现代植物育种在很大程度上依赖于分子标记的使用。近年来，下一代测序（NGS）作为一种强大的技术出现，可非常快速且经济有效地发现 DNA 序列多态性并生成分子标记，从而加速植物育种计划。番茄中的单个显性基因 Frl 提供对真菌病原体尖孢镰刀菌（Fusarium oxysporum f. sp. radicis-lycopersici，简称 FORL）的抗性，该病原体是枯萎病和根腐病的致病因子。在本研究中，我们描述了与 Frl 基因座相关的分子标记的产生。在抗 FORL 和易感品种之间产生了一个 F 作图群体。然后使用 NGS 技术对一个易感和一个抗性亲本的基因组以及混池的抗性和易感 F 系的基因组进行测序。我们将研究重点放在 Frl 基因座上，并将该基因座定位到 9 号染色体上的 900 kb 区间内。在该区间内鉴定出了多态性单核苷酸多态性（SNP），并生成了与抗性表型共分离的标记。其中一些标记已成功用于商业番茄品种的测试，表明它们可用于大规模育种计划中的标记辅助选择。
