Kahveci Erdem, Devran Zübeyir, Özkaynak Ercan, Hong Yiguo, Studholme David J, Tör Mahmut
M.Y. Genetik Tarim Tek. Lab. Tic. Ltd. Sti., Antalya, Turkey.
Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, Antalya, Turkey.
Front Plant Sci. 2021 Aug 18;12:691576. doi: 10.3389/fpls.2021.691576. eCollection 2021.
Cucumber is a widely grown vegetable crop plant and a host to many different plant pathogens. (CVYV) causes economic losses on cucumber crops in Mediterranean countries and in some part of India such as West Bengal and in African countries such as Sudan. CVYV is an RNA potyvirus transmitted mechanically and by whitefly () in a semipersistent manner. Control of this virus is heavily dependent on the management of the insect vector and breeding virus-resistant lines. DNA markers have been used widely in conventional plant breeding programs marker-assisted selection (MAS). However, very few resistance sources against CVYV in cucumber exist, and also the lack of tightly linked molecular markers to these sources restricts the rapid generation of resistant lines. In this work, we used genomics coupled with the bulked segregant analysis method and generated the MAS-friendly Kompetitive allele specific PCR (KASP) markers suitable for selection in cucumber breeding using a segregating F mapping population and commercial plant lines. Variant analysis was performed to generate single-nucleotide polymorphism (SNP)-based markers for mapping the population and genotyping the commercial lines. We fine-mapped the region by generating new markers down to 101 kb with eight genes. We provided SNP data for this interval, which could be useful for breeding programs and cloning the candidate genes.
黄瓜是一种广泛种植的蔬菜作物，也是许多不同植物病原体的宿主。黄瓜黄化脉斑驳病毒（CVYV）在地中海国家以及印度的一些地区（如西孟加拉邦）和非洲国家（如苏丹）给黄瓜作物造成经济损失。CVYV是一种RNA马铃薯Y病毒，通过机械传播以及由烟粉虱以半持久性方式传播。对这种病毒的控制在很大程度上依赖于对昆虫媒介的管理以及培育抗病毒品系。DNA标记已广泛用于传统植物育种计划中的标记辅助选择（MAS）。然而，黄瓜中针对CVYV的抗性来源非常少，而且与这些来源紧密连锁的分子标记的缺乏限制了抗性品系的快速培育。在这项工作中，我们结合基因组学和混合分组分析法，利用分离的F作图群体和商业植物品系，生成了适用于黄瓜育种选择的对MAS友好的竞争性等位基因特异性PCR（KASP）标记。进行变异分析以生成基于单核苷酸多态性（SNP）的标记，用于群体作图和商业品系的基因分型。我们通过生成新的标记将区域精细定位到101 kb，其中包含八个基因。我们提供了该区间的SNP数据，这可能对育种计划和克隆候选基因有用。
