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使用表面增强拉曼散射作为急性肾移植排斥反应的预后指标。

Use of surface-enhanced Raman scattering as a prognostic indicator of acute kidney transplant rejection.

作者信息

Chi Jingmao, Zaw Thet, Cardona Iliana, Hosnain Mujtaba, Garg Neha, Lefkowitz Heather R, Tolias Peter, Du Henry

机构信息

Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ, 07030, USA.

Newark Beth Israel Medical Center, Newark, NJ, 07112, USA.

出版信息

Biomed Opt Express. 2015 Feb 10;6(3):761-9. doi: 10.1364/BOE.6.000761. eCollection 2015 Mar 1.

Abstract

We report an early, noninvasive and rapid prognostic method of predicting potential acute kidney dysfunction using surface-enhanced Raman scattering (SERS). Our analysis was performed on urine samples collected prospectively from 58 kidney transplant patients using a He-Ne laser (632.8 nm) as the excitation source. All abnormal kidney function episodes (three acute rejections and two acute kidney failures that were eventually diagnosed independently by clinical biopsy) consistently exhibited unique SERS spectral features in just one day following the transplant surgery. These results suggested that SERS analysis provides an early and more specific indication to kidney function than the clinically used biomarker, serum creatinine (sCr).

摘要

我们报告了一种使用表面增强拉曼散射(SERS)预测潜在急性肾功能障碍的早期、非侵入性且快速的预后方法。我们使用氦氖激光(632.8 nm)作为激发源,对前瞻性收集的58例肾移植患者的尿液样本进行了分析。所有肾功能异常事件(3例急性排斥反应和2例急性肾衰竭,最终通过临床活检独立诊断)在移植手术后仅一天就一致表现出独特的SERS光谱特征。这些结果表明,与临床使用的生物标志物血清肌酐(sCr)相比,SERS分析能为肾功能提供更早且更具特异性的指标。

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本文引用的文献

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Nephron Clin Pract. 2011;118(2):c173-81; discussion c181. doi: 10.1159/000321381. Epub 2010 Dec 16.
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Localized surface plasmon resonance spectroscopy and sensing.局域表面等离子体共振光谱学与传感
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Free heme toxicity and its detoxification systems in human.人体内的游离血红素毒性及其解毒系统。
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Early versus late acute rejection episodes in renal transplantation.肾移植中早期与晚期急性排斥反应
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