Chi Jingmao, Zaw Thet, Cardona Iliana, Hosnain Mujtaba, Garg Neha, Lefkowitz Heather R, Tolias Peter, Du Henry
Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ, 07030, USA.
Newark Beth Israel Medical Center, Newark, NJ, 07112, USA.
Biomed Opt Express. 2015 Feb 10;6(3):761-9. doi: 10.1364/BOE.6.000761. eCollection 2015 Mar 1.
We report an early, noninvasive and rapid prognostic method of predicting potential acute kidney dysfunction using surface-enhanced Raman scattering (SERS). Our analysis was performed on urine samples collected prospectively from 58 kidney transplant patients using a He-Ne laser (632.8 nm) as the excitation source. All abnormal kidney function episodes (three acute rejections and two acute kidney failures that were eventually diagnosed independently by clinical biopsy) consistently exhibited unique SERS spectral features in just one day following the transplant surgery. These results suggested that SERS analysis provides an early and more specific indication to kidney function than the clinically used biomarker, serum creatinine (sCr).
我们报告了一种使用表面增强拉曼散射（SERS）预测潜在急性肾功能障碍的早期、非侵入性且快速的预后方法。我们使用氦氖激光（632.8 nm）作为激发源，对前瞻性收集的58例肾移植患者的尿液样本进行了分析。所有肾功能异常事件（3例急性排斥反应和2例急性肾衰竭，最终通过临床活检独立诊断）在移植手术后仅一天就一致表现出独特的SERS光谱特征。这些结果表明，与临床使用的生物标志物血清肌酐（sCr）相比，SERS分析能为肾功能提供更早且更具特异性的指标。
