折射率与线粒体活性之间的显著相关性：单个线粒体研究
Significant correlation between refractive index and activity of mitochondria: single mitochondrion study.
作者信息
Haseda Keisuke, Kanematsu Keita, Noguchi Keiichi, Saito Hiromu, Umeda Norihiro, Ohta Yoshihiro
机构信息
Division of Biotechnology and Life Sciences, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
Instrumentation Analysis Center, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
出版信息
Biomed Opt Express. 2015 Feb 19;6(3):859-69. doi: 10.1364/BOE.6.000859. eCollection 2015 Mar 1.
Abstract
Measurements of refractive indices (RIs) of intracellular components can provide useful information on the structure and function of cells. The present study reports, for the first time, determination of the RI of an isolated mitochondrion in isotonic solution using retardation-modulated differential interference contrast microscopy. The value was 1.41 ± 0.01, indicating that mitochondria are densely packed with molecules having high RIs. Further, the RIs of each mitochondrion were significantly correlated with the mitochondrial membrane potential, an index of mitochondrial activity. These results will provide useful information on the structures and functions of cells based on the intracellular distribution of RIs.
摘要
对细胞内组分的折射率（RI）进行测量能够提供有关细胞结构和功能的有用信息。本研究首次报道了使用延迟调制微分干涉对比显微镜测定等渗溶液中分离线粒体的RI。该值为1.41±0.01，表明线粒体中密集地充满了具有高RI的分子。此外，每个线粒体的RI与线粒体膜电位（线粒体活性指标）显著相关。这些结果将基于RI的细胞内分布提供有关细胞结构和功能的有用信息。
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