Keahey P A, Tkaczyk T S, Schmeler K M, Richards-Kortum R R
Department of Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77025 USA.
Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, 1100 Holcombe Boulevard, Houston, TX 77030 USA.
Biomed Opt Express. 2015 Feb 19;6(3):870-80. doi: 10.1364/BOE.6.000870. eCollection 2015 Mar 1.
Fiber-optic microendoscopes have shown promise to image the changes in nuclear morphometry that accompany the development of precancerous lesions in tissue with squamous epithelium such as in the oral mucosa and cervix. However, fiber-optic microendoscopy image contrast is limited by out-of-focus light generated by scattering within tissue. The scattering coefficient of tissues with columnar epithelium can be greater than that of squamous epithelium resulting in decreased image quality. To address this challenge, we present a small and portable microendoscope system capable of performing optical sectioning using structured illumination (SI) in real-time. Several optical phantoms were developed and used to quantify the sectioning capabilities of the system. Columnar epithelium from cervical tissue specimens was then imaged ex vivo, and we demonstrate that the addition of SI achieves higher image contrast, enabling visualization of nuclear morphology.
光纤微型内窥镜已显示出有望对伴有癌前病变发展的核形态测量变化进行成像，这些病变发生在具有鳞状上皮的组织中，如口腔黏膜和子宫颈。然而，光纤微型内窥镜图像对比度受到组织内散射产生的离焦光的限制。具有柱状上皮的组织的散射系数可能大于鳞状上皮的散射系数，从而导致图像质量下降。为应对这一挑战，我们展示了一种小型便携式微型内窥镜系统，该系统能够使用结构光照明（SI）实时进行光学切片。开发了几种光学模型并用于量化该系统的切片能力。然后对宫颈组织标本的柱状上皮进行离体成像，我们证明添加SI可实现更高的图像对比度，从而能够可视化核形态。
