Rajendran Peramaiyan, Rengarajan Thamaraiselvan, Nishigaki Yutaka, Palaniswami Rajendran, Nishigaki Ikuo
a NPO-International Laboratory of Biochemistry , Nakagawa-ku , Nagoya , Japan and.
b Department of Applied Zoology and Biotechnology , Vivekananda College, Affiliated to Madurai Kamaraj University , Thiruvedakam West, Madurai , India.
J Recept Signal Transduct Res. 2016;36(1):57-66. doi: 10.3109/10799893.2015.1019137. Epub 2015 Mar 23.
The therapeutic effects of the natural antioxidant mangiferin (a xanthonoid and potent oxygen free radical scavenger), which is widely distributed in mango fruit, against CdCl(2)-induced toxicity in human renal glomerulus endothelial cells (HRGEC) were investigated. The viability of HREGCs that were treated with CdCl(2) (25 µ mol) and co-treated with mangiferin (75 µ mol) for 24 h was measured by crystal violet dye. The exposure of human glomerulus renal endothelial cells to cadmium promotes a polarized apical secretion of IL-6 and IL-8, two pivotal proinflammatory cytokines known to play a significant role in renal inflammation. Proinflammatory cytokine secretion by human renal glomerulus endothelial cells could be the result of cadmium-induced IL-6 secretion via an NF-κB-dependent pathway. However, IL-8 secretion involves the phosphor-JNK phospho-p38 signaling pathway. The results of the current study reveal that mangiferin could prevent both cadmium-induced IL-6 and IL-8 secretion by human glomerulus endothelial cells and be used to prevent renal inflammation.
天然抗氧化剂芒果苷（一种呫吨酮类化合物和强效氧自由基清除剂，广泛分布于芒果果实中）对氯化镉诱导的人肾小球内皮细胞（HRGEC）毒性的治疗作用进行了研究。用结晶紫染料测定了用氯化镉（25 µmol）处理并与芒果苷（75 µmol）共同处理24小时的人肾小球内皮细胞的活力。人肾小球肾内皮细胞暴露于镉会促进白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的极化顶端分泌，这两种关键的促炎细胞因子在肾脏炎症中起重要作用。人肾小球内皮细胞分泌促炎细胞因子可能是镉通过NF-κB依赖性途径诱导IL-6分泌的结果。然而，IL-8的分泌涉及磷酸化-应激活化蛋白激酶（phosphor-JNK）磷酸化-p38信号通路。当前研究结果表明，芒果苷可以预防镉诱导的人肾小球内皮细胞分泌IL-6和IL-8，并可用于预防肾脏炎症。
