Le Gal Solène, Pougnet Laurence, Damiani Céline, Fréalle Emilie, Guéguen Paul, Virmaux Michèle, Ansart Séverine, Jaffuel Sylvain, Couturaud Francis, Delluc Aurélien, Tonnelier Jean-Marie, Castellant Philippe, Le Meur Yann, Le Floch Gaétan, Totet Anne, Menotti Jean, Nevez Gilles
University of Brest, LUBEM EA 3882, SFR 148, Brest, France; Laboratory of Parasitology and Mycology, Brest University Hospital, Brest, France.
Laboratory, Military Teaching Hospital Clermont Tonnerre, Brest, France.
Diagn Microbiol Infect Dis. 2015 Jun;82(2):137-42. doi: 10.1016/j.diagmicrobio.2015.01.004. Epub 2015 Jan 15.
In this study, Pneumocystis jirovecii was detected and characterized in the air surrounding patients with Pneumocystis pulmonary colonization. Air samples were collected in the rooms of 10 colonized patients using Coriolis® μ air sampler at 1m and 5m from the patient's head. P. jirovecii DNA was amplified and genotyped in pulmonary and air samples at the mitochondrial large subunit ribosomal RNA gene. P. jirovecii DNA was detected in 5 of the 10 air samples collected at 1m and in 5 of the 10 other air samples collected at 5m. P. jirovecii genotyping was successful in 4 pairs or triplets of air and pulmonary samples. Full genotype matches were observed in 3 of the 4 pairs or triplets of air and pulmonary samples. These results provide original data supporting P. jirovecii exhalation from colonized patients and emphasize the risk of P. jirovecii nosocomial transmission from this patient population.
在本研究中，对肺孢子菌肺定植患者周围空气中的耶氏肺孢子菌进行了检测和特征分析。使用科里奥利μ空气采样器在10例定植患者房间内距患者头部1米和5米处采集空气样本。在肺部和空气样本中，对线粒体大亚基核糖体RNA基因的耶氏肺孢子菌DNA进行扩增和基因分型。在1米处采集的10份空气样本中有5份检测到耶氏肺孢子菌DNA，在5米处采集的另外10份空气样本中有5份检测到该菌DNA。在4对或4组空气和肺部样本中成功进行了耶氏肺孢子菌基因分型。在4对或4组空气和肺部样本中有3对或3组观察到完全基因匹配。这些结果提供了原始数据，支持耶氏肺孢子菌从定植患者呼出，并强调了该患者群体中耶氏肺孢子菌医院内传播的风险。
