Krause C, Ens K, Fechner K, Voigt J, Fraune J, Rohwäder E, Hahn M, Danckwardt M, Feirer C, Barth E, Martinetz T, Stöcker W
Institute for Experimental Immunology, Euroimmun AG, Lübeck, Germany.
Institute for Experimental Immunology, Euroimmun AG, Lübeck, Germany
Lupus. 2015 Apr;24(4-5):516-29. doi: 10.1177/0961203314559635.
Antinuclear autoantibodies (ANA) are highly informative biomarkers in autoimmune diagnostics. The increasing demand for effective test systems, however, has led to the development of a confusingly large variety of different platforms. One of them, the indirect immunofluorescence (IIF), is regarded as the common gold standard for ANA screening, as described in a position statement by the American College of Rheumatology in 2009. Technological solutions have been developed aimed at standardization and automation of IIF to overcome methodological limitations and subjective bias in IIF interpretation. In this review, we present the EUROPattern Suite, a system for computer-aided immunofluorescence microscopy (CAIFM) including automated acquisition of digital images and evaluation of IIF results. The system was originally designed for ANA diagnostics on human epithelial cells, but its applications have been extended with the latest system update version 1.5 to the analysis of antineutrophil cytoplasmic antibodies (ANCA) and anti-dsDNA antibodies.
抗核自身抗体（ANA）是自身免疫诊断中极具信息价值的生物标志物。然而，对有效检测系统的需求不断增加，导致开发出了种类繁多、令人困惑的不同平台。其中之一，间接免疫荧光法（IIF），被视为ANA筛查的通用金标准，如美国风湿病学会2009年的一份立场声明中所述。为克服IIF解释中的方法学局限性和主观偏差，已开发出旨在实现IIF标准化和自动化的技术解决方案。在本综述中，我们介绍了EUROPattern Suite，这是一种用于计算机辅助免疫荧光显微镜检查（CAIFM）的系统，包括数字图像的自动采集和IIF结果评估。该系统最初设计用于人上皮细胞的ANA诊断，但随着最新的1.5版系统更新，其应用已扩展到抗中性粒细胞胞浆抗体（ANCA）和抗双链DNA抗体的分析。
