Johnson L D, Idler W W, Zhou X M, Roop D R, Steinert P M
Proc Natl Acad Sci U S A. 1985 Apr;82(7):1896-900. doi: 10.1073/pnas.82.7.1896.
We present the structure and nucleotide sequence of a gene encoding the human epidermal 67-kDa keratin. Three genomic clones were isolated from a lambda Charon 4A human genomic library by hybridization to a specific cDNA probe. One clone of 12.3 kilobase pairs was shown by R-loop, DNA sequence, and primer-extension analyses to encode an entire gene of about 6.25 kilobase pairs. Of eight identified introns, seven are located within the region that encodes the central coiled-coil alpha-helical domain of the protein. Except for one intron located at the end of the region encoding this domain, these do not delineate apparent structural subdomains. The positions of five of the introns exactly coincide with the positions of introns previously reported in the hamster gene for the intermediate filament protein vimentin [Quax, W., Egberts, W.V., Hendricks, W., Quax-Jeuken, Y. & Bloemandal, H. (1983) Cell 35, 215-233]. These findings suggest that the human 67-kDa keratin and vimentin genes arose from a common ancestral gene.
我们展示了编码人表皮67 kDa角蛋白的基因的结构和核苷酸序列。通过与特定的cDNA探针杂交，从λ噬菌体Charon 4A人基因组文库中分离出三个基因组克隆。通过R环、DNA序列和引物延伸分析表明，一个12.3千碱基对的克隆编码一个约6.25千碱基对的完整基因。在八个已鉴定的内含子中，有七个位于编码该蛋白质中央卷曲螺旋α螺旋结构域的区域内。除了位于该结构域编码区域末端的一个内含子外，这些内含子并没有划分出明显的结构亚域。其中五个内含子的位置与先前报道的仓鼠中间丝蛋白波形蛋白基因中的内含子位置完全一致[夸克斯，W.，埃格伯茨，W.V.，亨德里克斯，W.，夸克斯-耶肯，Y. & 布洛门达尔，H.（1983年）《细胞》35卷，215 - 233页]。这些发现表明，人67 kDa角蛋白基因和波形蛋白基因起源于一个共同的祖先基因。
