Bhattacharyya Kiran, Eshein Adam, Chandrasekhar Anand, Viator John A
Department of Biomedical Engineering, Northwestern University, Evanston, IL 60201, USA.
Phys Med Biol. 2015 Apr 21;60(8):3081-96. doi: 10.1088/0031-9155/60/8/3081. Epub 2015 Mar 24.
Photoacoustics can be used as a label-free spectroscopic method of identifying pigmented proteins and characterizing their intracellular concentration over time in a single living cell. The authors use a microscopic laser irradiation system with a 5 ns, Q-switched laser focused onto single cells in order to collect photoacoustic responses of melanoma cells from the HS936 cell line and gold nanoparticle labeled breast cancer cells from the T47D cell line. The volume averaged intracellular concentration of melanin is found to range from 29-270 mM for single melanoma cells and the number of gold nanoparticles (AuNP) is shown to range from 850-5900 AuNPs/cell. Additionally, the melanin production response to UV-A light stimulus is measured in four melanoma cells to find a mass production rate of 5.7 pg of melanin every 15 min.
光声技术可作为一种无标记光谱方法，用于识别色素蛋白并随时间表征其在单个活细胞内的浓度。作者使用了一个微观激光照射系统，该系统配备一个5纳秒的调Q激光器，聚焦于单个细胞，以便收集来自HS936细胞系的黑色素瘤细胞和来自T47D细胞系的金纳米颗粒标记的乳腺癌细胞的光声响应。发现单个黑色素瘤细胞中黑色素的体积平均细胞内浓度范围为29 - 270毫摩尔，金纳米颗粒（AuNP）的数量范围为850 - 5900个/细胞。此外，在四个黑色素瘤细胞中测量了黑色素对UV - A光刺激的产生反应，发现每15分钟黑色素的大量产生速率为5.7皮克。
