Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, 23071, Jaén, Spain.
Cellular Biology Section, Department of Experimental Biology, University of Jaén, Jaén, Spain.
Eur J Nutr. 2016 Mar;55(2):639-650. doi: 10.1007/s00394-015-0884-3. Epub 2015 Mar 25.
We have previously reported that tyrosol (TYR) promotes lifespan extension in the nematode Caenorhabditis elegans, also inducing a stronger resistance to thermal and oxidative stress in vivo. In this study, we performed a whole-genome DNA microarray in order to narrow down the search for candidate genes or signaling pathways potentially involved in TYR effects on C. elegans longevity.
Nematodes were treated with 0 or 250 μM TYR, total RNA was isolated at the adult stage, and derived cDNA probes were hybridized to Affymetrix C. elegans expression arrays. Microarray data analysis was performed, and relative mRNA expression of selected genes was validated using qPCR.
Microarray analysis identified 208 differentially expressed genes (206 over-expressed and two under-expressed) when comparing TYR-treated nematodes with vehicle-treated controls. Many of these genes are linked to processes such as regulation of growth, transcription, reproduction, lipid metabolism and body morphogenesis. Moreover, we detected an interesting overlap between the expression pattern elicited by TYR and those induced by other dietary polyphenols known to extend lifespan in C. elegans, such as quercetin and tannic acid.
Our results suggest that important cellular mechanisms directly related to longevity are influenced by TYR treatment in C. elegans, supporting our previous notion that this phenol might act on conserved genetic pathways to increase lifespan in a whole organism.
我们之前报道过酪醇（TYR）能延长秀丽隐杆线虫的寿命，还能增强其体内对热应激和氧化应激的抵抗力。在这项研究中，我们进行了全基因组 DNA 微阵列分析，以缩小候选基因或信号通路的搜索范围，这些基因或信号通路可能与 TYR 对秀丽隐杆线虫寿命的影响有关。
用 0 或 250 μM TYR 处理线虫，在成虫期提取总 RNA，并将衍生的 cDNA 探针与 Affymetrix 秀丽隐杆线虫表达阵列杂交。进行微阵列数据分析，并使用 qPCR 验证所选基因的相对 mRNA 表达。
与用对照溶剂处理的线虫相比，比较 TYR 处理的线虫时，微阵列分析鉴定出 208 个差异表达的基因（206 个上调和两个下调）。这些基因中有许多与生长、转录、繁殖、脂质代谢和身体形态发生等过程有关。此外，我们还检测到 TYR 诱导的表达模式与其他已知能延长秀丽隐杆线虫寿命的饮食多酚（如槲皮素和鞣酸）诱导的表达模式之间存在有趣的重叠。
我们的结果表明，TYR 处理会影响秀丽隐杆线虫中与寿命直接相关的重要细胞机制，这支持了我们之前的观点，即这种酚类物质可能作用于保守的遗传途径，以增加整个生物体的寿命。
