Chen Min, Ma Yanling, Yang Chaojun, Yang Li, Chen Huichao, Dong Lijuan, Dai Jie, Jia Manhong, Lu Lin
Center for AIDS/STD Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming, Yunnan 650022, China.
Center for AIDS/STD Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming, Yunnan 650022, China; College of Public Health, Kunming Medical University, Kunming, Yunnan 650500, China.
PLoS One. 2015 Mar 25;10(3):e0119989. doi: 10.1371/journal.pone.0119989. eCollection 2015.
To investigate the linkage of HIV transmission from a man to a woman through unprotected sexual contact without disclosing his HIV-positive status.
Combined with epidemiological information and serological tests, phylogenetic analysis was used to test the a priori hypothesis of HIV transmission from the man to the woman. Control subjects, infected with HIV through heterosexual intercourse, from the same location were also sampled. Phylogenetic analyses were performed using the consensus gag, pol and env sequences obtained from blood samples of the man, the woman and the local control subjects. The env quasispecies of the man, the woman, and two controls were also obtained using single genome amplification and sequencing (SGA/S) to explore the paraphyletic relationship by phylogenetic analysis.
Epidemiological information and serological tests indicated that the man was infected with HIV-1 earlier than the woman. Phylogenetic analyses of the consensus sequences showed a monophyletic cluster for the man and woman in all three genomic regions. Furthermore, gag sequences of the man and woman shared a unique recombination pattern from subtype B and C, which was different from those of CRF07_BC or CRF08_BC observed in the local samples. These indicated that the viral sequences from the two subjects display a high level of similarity. Further, viral quasispecies from the man exhibited a paraphyletic relationship with those from the woman in the Bayesian and maximum-likelihood (ML) phylogenetic trees of the env region, which supported the transmission direction from the man to the woman.
In the context of epidemiological and serological evidence, the results of phylogenetic analyses support the transmission from the man to the woman.
调查一名男子在未披露其HIV阳性状态的情况下，通过无保护性行为将HIV传播给一名女性的关联性。
结合流行病学信息和血清学检测，采用系统发育分析来检验该男子将HIV传播给该女性的先验假设。还对来自同一地点、通过异性性行为感染HIV的对照对象进行了采样。使用从该男子、该女性和当地对照对象的血样中获得的gag、pol和env序列共识进行系统发育分析。还通过单基因组扩增和测序（SGA/S）获得了该男子、该女性和两名对照的env准种，以通过系统发育分析探索并系关系。
流行病学信息和血清学检测表明，该男子感染HIV-1的时间早于该女性。对共识序列的系统发育分析显示，在所有三个基因组区域中，该男子和该女性形成一个单系簇。此外，该男子和该女性的gag序列共享一种独特的B亚型和C亚型重组模式，这与在当地样本中观察到的CRF07_BC或CRF08_BC不同。这些表明来自这两名受试者的病毒序列显示出高度相似性。此外，在env区域的贝叶斯和最大似然（ML）系统发育树中，该男子的病毒准种与该女性的病毒准种呈现并系关系，这支持了从该男子到该女性的传播方向。
在流行病学和血清学证据的背景下，系统发育分析结果支持从该男子到该女性的传播。
