Kolek Jan, Patáková Petra, Melzoch Karel, Sigler Karel, Řezanka Tomáš
Department of Biotechnology, University of Chemistry and Technology Prague, Prague, Czech Republic.
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
PLoS One. 2015 Mar 25;10(3):e0122058. doi: 10.1371/journal.pone.0122058. eCollection 2015.
Changes in membrane lipid composition of Clostridium pasteurianum NRRL B-598 were studied during butanol fermentation by lipidomic analysis, performed by high resolution electrospray ionization tandem mass spectrometry. The highest content of plasmalogen phospholipids correlated with the highest butanol productivity, which indicated a probable role of these compounds in the complex responses of cells toward butanol stress. A difference in the ratio of saturated to unsaturated fatty acids was found between the effect of butanol produced by the cells and butanol added to the medium. A decrease in the proportion of saturated fatty acids during conventional butanol production was observed while a rise in the content of these acids appeared when butanol was added to the culture. The largest change in total plasmalogen content was observed one hour after butanol addition i.e. at the 7th hour of cultivation. When butanol is produced by bacterial cells, then the cells are not subjected to severe stress and responded to it by relatively slowly changing the content of fatty acids and plasmalogens, while after a pulse addition of external butanol (to a final non-lethal concentration of 0.5 % v/v) the cells reacted relatively quickly (within a time span of tens of minutes) by increasing the total plasmalogen content.
通过高分辨率电喷雾电离串联质谱法进行脂质组学分析，研究了巴斯德梭菌NRRL B - 598在丁醇发酵过程中膜脂组成的变化。缩醛磷脂的最高含量与最高的丁醇生产率相关，这表明这些化合物在细胞对丁醇胁迫的复杂反应中可能发挥作用。在细胞产生的丁醇的影响和添加到培养基中的丁醇之间，发现了饱和脂肪酸与不饱和脂肪酸比例的差异。在传统丁醇生产过程中观察到饱和脂肪酸比例下降，而当向培养物中添加丁醇时，这些酸的含量则会上升。在添加丁醇后一小时即培养的第7小时观察到缩醛磷脂总含量的最大变化。当细菌细胞产生丁醇时，细胞不会受到严重胁迫，而是通过相对缓慢地改变脂肪酸和缩醛磷脂的含量来做出反应，而在脉冲添加外部丁醇（至最终非致死浓度0.5% v/v）后，细胞通过增加缩醛磷脂总含量相对快速地（在几十分钟的时间范围内）做出反应。
