Brade L, Nurminen M, Mäkelä P H, Brade H
Infect Immun. 1985 May;48(2):569-72. doi: 10.1128/iai.48.2.569-572.1985.
The antigenic properties of the lipopolysaccharide (LPS) of Chlamydia trachomatis L2 were investigated. By means of passive hemolysis, passive hemolysis inhibition, and absorption experiments, it was shown that antiserum raised against chlamydial elementary bodies contained at least two different antibody specificities which reacted with different antigenic determinants of chlamydial LPS. One of these antibodies cross-reacted with enterobacterial Re LPS, recognizing a structure which is shared by both LPSs, whereas the reactivity of the second antibody was restricted to chlamydial LPS. The former antibody could be absorbed with Salmonella minnesota Re LPS, whereas the latter was not affected by this absorption. Therefore, chlamydial LPS possesses two distinct antigenic determinants, one of which is C. trachomatis specific, the other of which is responsible for the cross-reactivity with enterobacterial Re-type LPS. Both antigenic determinants were destroyed during mild acid-catalyzed hydrolysis. It was further shown that free chlamydial lipid A exhibits antigenicity that cross-reacts with free enterobacterial lipid A. This antigenicity, however, as in enterobacterial LPS, is present in a cryptic form, i.e., it is unmasked only after acid hydrolysis of LPS.
对沙眼衣原体L2脂多糖（LPS）的抗原特性进行了研究。通过被动溶血、被动溶血抑制和吸收实验表明，针对衣原体原体产生的抗血清含有至少两种不同的抗体特异性，它们与衣原体LPS的不同抗原决定簇发生反应。其中一种抗体与肠杆菌属Re LPS发生交叉反应，识别两种LPS共有的一种结构，而第二种抗体的反应性则仅限于衣原体LPS。前一种抗体可被明尼苏达沙门氏菌Re LPS吸收，而后者不受这种吸收的影响。因此，衣原体LPS具有两种不同的抗原决定簇，其中一种是沙眼衣原体特异性的，另一种则负责与肠杆菌属Re型LPS的交叉反应。两种抗原决定簇在温和酸催化水解过程中均被破坏。进一步表明，游离的衣原体脂质A表现出与游离肠杆菌脂质A发生交叉反应的抗原性。然而，与肠杆菌LPS一样，这种抗原性以隐蔽形式存在，即仅在LPS酸水解后才会暴露出来。
